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大豆科学  2015 

光谱分布对大豆子叶节再生的影响

DOI: 10.11861/j.issn.1000-9841.2015.05.0826, PP. 826-832

Keywords: 大豆,光谱分布,子叶节,丛生芽,LED

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Abstract:

以辽鲜1号大豆品种为试材,研究了不同光谱分布对大豆子叶节再生的影响,为菜用大豆子叶节遗传转化受体体系的改进提供借鉴。以萌发7d的子叶节为外植体,依次接种于丛生芽诱导培养基、伸长培养基和生根培养基中,并且分别置于6种LED组合光源中培育,以荧光灯作为对照。光密度设置为50μmol.m-2.s-1,光周期设置为12h.d-1。结果表明:复合光谱中630nm红光会抑制丛生芽之间相互伸长,而660nm红光能显著缓解相互抑制作用,并且更有利于丛生芽形态生长、叶绿素合成和干物质积累以及生根苗形态生长、干物质积累和根系发育。添加绿光或黄光均抑制丛生芽相互之间的伸长,且添加黄光抑制更强,添加绿光可促进丛生芽的叶绿素合成、干物质积累及生根壮苗。

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