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分析化学  2015 

超高效液相色谱快速分析不同哺乳动物胆汁中胆汁酸

DOI: 10.11895/j.issn.0253-3820.150118, PP. 675-681

Keywords: 超高速液相色谱,哺乳动物,胆汁酸,胆结石

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Abstract:

建立超高效液相色谱同时快速测定胆汁中13种胆汁酸浓度的方法,并对种内种间差异进行统计学分析。色谱柱为KyotoC18(100mm×2.1mm,2.2μm);流动相为乙腈-10mmol/LKH2PO4溶液,梯度洗脱;流速:0.5mL/min;检测波长200nm。各种胆汁酸在各自测定浓度范围内与峰面积的线性关系良好(r>0.9995);所有胆汁酸能够达到良好分离。13种胆汁酸检出限(S/N=3)在0.2~0.6ng/μL之间。与传统HPLC法相比,本方法简便、快速,精密度高,重现性好,适用于胆汁酸的分析。

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