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高产二羟基丙酮重组菌株的构建

DOI: 10.13560/j.cnki.biotech.bull.1985.2015.08.025, PP. 174-179

Keywords: 1,3-二羟基丙酮,重组菌株,甘油,过量表达

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Abstract:

旨在构建一株过量表达编码膜系甘油脱氢酶的sldAB基因的重组菌株,以提高二羟基丙酮产量。以氧化葡萄糖酸杆菌ATCC621H的基因组DNA为模板,运用PCR方法扩增得到基因sldAB,并连接到pBBR1MCS-2质粒上,构建表达载体pBBR1MCS-2-sldAB。通过电转化将载体pBBR1MCS-2-sldAB转化进入氧化葡萄糖酸杆菌ATCC621H内,得到重组菌株GOX205。结果显示,重组菌株构建成功,其甘油脱氢酶的酶活力较之于出发菌株提高了26%。在甘油初始浓度100g/L的甘油发酵培养基中,较之于出发菌株,GOX205的生长状况良好,发酵52h时DHA浓度达到94.1g/L,较之于出发菌株提高了19.7%,甘油残量降低了15.1g/L。

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