|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
药用真菌猪苓4种小gtpase基因的克隆和表达分析
, PP. 1186-1191
Keywords: 猪苓,小gtpase,菌核发育
Abstract:
利用race-pcr技术首次从药用真菌猪苓中分离得到猪苓菌核的4个小gtpase基因。这4个基因分别命名为purhoa1、purhoa2、puypt1及puras,purhoa1全长698bp,编码区共585bp,编码194个氨基酸,推测分子量为21.75kd,理论等电点为6.44;purhoa2全长837bp,其中编码区长585bp,可编码194个氨基酸,计算其分子量为21.75kd,理论等电点为6.33;puypt1为一条896bp的cdna,编码区为615bp,可编码204个氨基酸,经计算分子量为22.556kd,理论等电点为5.75。puras的cdna全长为803bp,其中编码区长639bp,编码212个氨基酸,推测分子量为23.821kd,理论等电点为5.2。purhoa1具有法尼酰转移酶催化位点(ft-caax)和香叶烯基转移酶催化位点(ggt1-caax),而purhoa2仅具有香叶烯基转移酶催化位点(ggt1-caax);puypt1具有小gtpase家族中rab1-ypt1的完整保守结构域;puras具有法尼酰转移酶催化位点(ft-caax)。系统进化树结果显示猪苓的4个小gtpase都隶属于担子菌类群。实时荧光定量pcr分析结果表明在菌核形成初期puypt1、puras、purhoa1在菌核中表达量显著高于菌丝,而purhoa2在菌核中的表达量显著低于菌丝,说明这4个小gtpase基因可能参与了猪苓菌核的形态发生。
| [1] | takaiy,sasakit,matozakit.smallgtp-bindingproteins[j].physiolrev,2001,81:153-208.
|
| [2] | chenc,dickmanmb.campblocksmapkactivationandsclerotialdevelopmentviarap-1inapka-independentmannerinsclerotiniasclerotiorum[j].molmicrobiol,2005,55:299-311.
|
| [3] | xingym,zhanglc,lianghq,etal.sclerotialformationofpolyporusumbellatusbylowtemperaturetreatmentunderartificialconditions[j].plosone,2013,8:e56190.
|
| [4] | liuyy,guosx.nutritionalfactorsdeterminingsclerotialformationofpolyporusumbellatus[j].lettapplmicrobiol,2009,49:283-288.
|
| [5] | madaulep,axelr.anovelras-relatedgenefamily[j].cell,1985,41:31-40.
|
| [6] | xingym,chenj,songc,etal.noxgeneexpressionandcytochemicallocalizationofhydrogenperoxideinpolyporusumbellatussclerotialformation[j].intjmolsci,2013,14:22967-22981.
|
| [7] | halla.rhogtpasesandthecontrolofcellbehaviour[j].biochemsoctrans,2005,33:891-895.
|
| [8] | parkj,choihj,lees,etal.rac-relatedgtp-bindingproteininelicitor-inducedreactiveoxygengenerationbysuspension-culturedsoybeancells[j].plantphysiol,2000,124:725-732.
|
| [9] | boltonmd,thommabphj,nelsonbd.sclerotiniasclerotiorum(lib.)debary:biologyandmoleculartraitsofacosmopolitanpathogen[j].molplantpathol,2006,7:1-16.
|
| [10] | leeme,singhk,sniderj,etal.therho1gtpaseactstogetherwithavacuolarglutathiones-conjugatetransportertoprotectyeastcellsfromoxidativestress[j].genetics,2011,188:859-870.
|
| [11] | adachiy,shibaiy,mitsushitaj,etal.oncogenicrasupregulatesnadphoxidase1geneexpressionthroughmekerk-dependentphosphorylationofgata-6[j].oncogene,2008,27:4921-4932.
|
| [12] | lih,yaow,fuy,etal.denovoassemblyanddiscoveryofgenesthatareinvolvedindroughttoleranceintibetansophoramoorcroftiana[j].plosone,2015,10:e111054.
|
| [13] | wennerbergk,rossmankl,dercj.therassuperfamilyataglance[j].jcellsci,2005,118:843-846.
|
| [14] | zhaoy,xierm,chaox,etal.bioactivity-directedisolation,identificationofdiureticcompoundsfrompolyporusumbellatus[j].jethnopharmacol,2009,126:184-187.
|
| [15] | logemannj,schellj,willmitzerl.improvedmethodfortheisolationofrnafromplanttissues[j].analbiochem,1987,163:16-20.
|
| [16] | pfafflmw.anewmathematicalmodelforrelativequantificationinreal-timert-pcr[j].nucleicacidsres,2001,29:e45.
|
| [17] | jonesma,raymondmj,yangz,etal.nadphoxidasedependentreactiveoxygenspeciesformationrequiredforroothairgrowthdependsonropgtpase[j].jexpbot,2007,58:1261-1270.
|
| [18] | weyemiu,lagente-chevalliero,boufraqechm,etal.ros-generatingnadphoxidasenox4isacriticalmediatorinoncogenich-ras-induceddnadamageandsubsequentsenescence[j].oncogene,2012,31:1117-1129.
|
| [19] | kimhj,chenc,kabbagem,etal.identificationandcharacterizationofsclerotiniasclerotiorumnadphoxidases[j].applenvironmicrobiol,2011,77:7721-7729.
|
Full-Text
|
|
Contact Us
[email protected] QQ:3279437679 
WhatsApp +8615387084133
|
|
