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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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两种荧光波长cdses/zns-cooh合金量子点微核组学效应特征的比较研究
Keywords: 合金量子点,遗传毒性,微核组学效应,cdses/zns-cooh
Abstract:
？目的:比较两种荧光波长cdses/zns-cooh合金量子点对细胞微核组效应遗传毒作用的特征。方法:采用l5178y细胞胞质分裂阻滞微核细胞组学试验,490和540nm两种荧光波长的合金量子点cdses/zns-cooh受试浓度均为0.0625、0.125、0.25、0.5和0.1mg/ml,观察其微核组效应、剂量-效应和时间-效应关系。结果:与阴性对照组相比,两种波长的cdses/zns-cooh合金量子点在0.0625mg/ml剂量时均可诱导微核率增加(p<0.05);两者都能诱导ⅰ型微核、ⅱ型微核和核芽效应,但490nm合金量子点不能诱导核质桥效应,而540nm合金量子点能诱导核质桥效应。490nm合金量子点在0.0625mg/ml可诱导产生总微核、ⅰ型微核和核芽,而540nm合金量子点在此浓度仅可诱导产生ⅰ型微核;两者均随着剂量进一步增加诱导产生其他效应;各种微核组效应有明显的剂量-效应关系(p<0.05)。490nm量子点在9h首先出现核质桥,540nm量子点在9h首先出现总微核和ⅱ型微核数增加;随时间延长进一步出现其他效应,27h各效应值达到峰值,此后下降。结论:两种荧光波长cdses/zns-cooh合金量子点均可诱导微核组学效应,但在相同剂量的效应谱、剂量-效应和时间-效应方面均有差异,表明两者的遗传毒作用特点有差异。
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