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OALib Journal期刊
ISSN: 2333-9721
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乙肝病毒s蛋白对人精子氧化应激的影响
Keywords: 乙肝病毒s蛋白,氧化应激,活性氧,脂质过氧化
Abstract:
？目的:乙肝病毒(hbv)可通过血睾屏障将病毒dna整合到男性生殖细胞的基因组中,诱发多种染色体畸变,而且还能破坏精子线粒体功能,导致精子活力受损,受精率和受精指数下降,但其机制未明。本研究主要考察乙肝病毒s蛋白(hbs)对人精子氧化应激的影响。方法:人精子与不同浓度(0、25、50、100μg/ml)hbs共孵育3h后,用流式细胞术检测精子内活性氧(ros)的变化情况,应用酶标仪检测精子细胞内丙二醛(mda)含量和总抗氧化能力(tac)。结果:人精子与25~100μg/mlhbs共孵育3h后,ros阳性精子比率、人精子细胞膜mda水平显著高于对照组,而tac水平显著低于对照组(p<0.05或0.01)。mda水平以及ros阳性精子比率的升高与hbs呈剂量依赖关系(r0.62,r0.75;p均<0.01)。tac水平的降低与hbs呈剂量依赖关系(r-0.89;p<0.01)。结论:hbs可诱导人精子内ros升高、tac降低,从而产生氧化应激,使细胞膜脂质过氧化进而影响人精子的功能。
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