OALib Journal期刊
ISSN: 2333-9721
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苯并(a)芘染毒致人支气管上皮细胞的周期改变及bpde-dna加合物形成
Keywords: 苯并(a)芘,细胞周期,bpde-dna加合物,人支气管上皮细胞
Abstract:
?目的:通过观察苯并(a)芘(bap)染毒致人支气管上皮细胞16hbe细胞周期改变及bpde-dna加合物形成的情况,探讨dna损伤与细胞周期阻滞之间的关系。方法:用不同浓度bap(0、1、2、4、8、16、32μmol/l)染毒16hbe细胞24h,用16μmol/lbap染毒16hbe细胞不同时间(0、1、2、4、8、12、24h)以检测bap染毒16hbe细胞的剂量和时间效应。再根据上述结果选择16μmol/lbap染毒16hbe细胞4h后,恢复不同时间(0、1、2、4、8、12、24h),处理结束后采用流式细胞术检测细胞周期分布情况,酶联免疫法和荧光免疫组化法检测bpde-dna加合物表达。结果:与正常对照组相比,随着bap染毒浓度和染毒时间的增加,s期细胞所占比例均增加(ppppp>0.05)。随着染毒浓度增加和染毒时间延长,16hbe细胞内bpde-dna加合物含量逐渐增加,与正常对照组相比差异均有统计学意义(ppr2=0.386,p=0.01)。结论:bap染毒所致bpde-dna加合物的形成与s期阻滞密切相关。
References
[1] | kerley-hamiltonjs,traskhw,ridleycj,etal.inherentandbenzo[a]pyrene-induceddifferentialarylhydrocarbonreceptorsignalinggreatlyaffectslifespan,atherosclerosis,cardiacgeneexpression,andbodyandheartgrowthinmice[j].toxicolsci,2012,126(2):391-404.
|
[2] | zhul,luh,chens,etal.pollutionlevel,phasedistributionandsourceanalysisofpolycyclicaromatichydrocarbonsinresidentialairinhangzhou,china[j].jhazardmater,2009,162(2/3):1165-1170.
|
[3] | hamoucheneh,arltvm,giddingsi,etal.influenceofcellcycleonresponsesofmcf-7cellstobenzo[a]pyrene[j].bmcgenomics,2011,12:333-348.
|
[4] | sertics,pizzis,lazzarof,etal.nerandddr:classicalmusicwithnewinstruments[j].cellcycle,2012,11(4):668-674.
|
[5] | bailisjm,forsburgsl.it'sallinthetiming:linkingsphasetochromatinstructureandchromosomedynamics[j].cellcycle,2003,2(4):303-306.
|
[6] | clibywa,lewiska,lillykk,etal.sphaseandg2arrestsinducedbytopoisomeraseipoisonsaredependentonatrkinasefunction[j].jbiolchem,2002,277(2):1599-1606.
|
[7] | yangj,wangz,chenw,etal.roleofubiquitinproteinligasering2indnadamageofhumanbronchialepithelialcellsexposedtobenzo[a]pyrene[j].jbiochemmoltoxicol,2013,27(7):357-363.
|
[8] | raginad,crawfordke,etheredgeaa,etal.agaschromatography-isotopedilutionhigh-resolutionmassspectrometrymethodforquantificationofisomericbenzo[a]pyrenediolepoxidehemoglobinadductsinhumans[j].janaltoxicol,2008,32(9):728-736.
|
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