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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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低剂量工业射线探伤工人职业暴露的遗传损伤效应
Keywords: 低剂量电离辐射,工业x射线探伤,遗传损伤,线粒体dna
Abstract:
？目的:探讨低剂量电离辐射对工业射线探伤工人的遗传损伤效应。方法:选取重庆市某国有企业探伤工人31人为探伤组,招募年龄、性别构成和吸烟率无显著差异的健康服务行业人员49人作为对照组。采用热释光剂量计(tld)对探伤人员进行个体剂量监测。微量全血培养的胞质分裂阻滞微核试验检测染色体损伤。采用real-timepcr分析线粒体dna(mtdna)拷贝数和超螺旋构象,长链pcr分析mtdna的完整性,qrt-pcr分析线粒体转录因子a(tfam)和过氧化酶体增殖受体γ轴共活化因子1α(pgc-1α)的mrna水平。结果:探伤工人的辐射年有效剂量为(0.15±0.03)msv/a,范围为(0.12~0.21)msv/a。探伤组的微核率和核芽率均显著增加(p<0.01)。探伤组mtdna拷贝数(40.04±24.99)与对照组(24.86±19.14)比较显著增加(p<0.05),探伤工人的mtdna超螺旋构象有显著改变(p<0.05),探伤组mtdna完整性(95.10±9.54)与对照组(313.51±14.58)相比明显降低(p<0.01),探伤组的tfam和pgc-1α的mrna水平均显著升高(p<0.01)。结论:探伤工人的辐射年有效剂量均值符合国家规定的放射职业接触标准。电离辐射职业暴露可引起探伤工人明显的染色体损伤和mtdna损伤,需采取更安全有效的防护措施。
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