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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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bhas42细胞转化试验高通量检测方法的建立及应用
Keywords: 遗传毒性,bhas42细胞转化,促癌活性,非遗传毒性致癌物,染料木黄酮
Abstract:
？目的:建立bhas42细胞转化试验高通量检测方法,并应用于染料木黄酮(gen)潜在致癌性的检测中。方法:建立bhas42细胞转化试验方法,比较细胞灶法和h2o2法对结果判定的影响。细胞灶法试验组在第21天直接固定染色。h2o2法试验组在细胞接种后第19天采用h2o2处理,染色并测定d(450)以确定细胞转化率。计算转化率以验证两种方法的相关性。并通过细胞生长试验选择适宜的gen浓度进行细胞转化试验,h2o2法判定转化试验结果。结果:在细胞灶法中,启动试验3-甲基胆蒽(3-mca)组、促癌试验佛波醇酯(tpa)组的转化灶个数分别与启动、促癌试验dmso组相比明显升高(p2o2法中,启动试验3-mca组、促癌试验tpa组的转化灶个数、d(450)分别与启动、促癌试验dmso组相比均明显升高(pd(450)相对于阴性组有显著差异(p结论:成功建立了bhas42细胞转化试验的h2o2法并实现了高通量检测,该法进一步缩短了实验周期并提高了结果的客观性,适用于非遗传毒性致癌物的体外早期筛选。gen在促癌试验中呈阳性,但在启动试验中呈阴性,提示其可能是非遗传毒性致癌物。
| [1] | sasakik.amethodforselectingmammaltransformedcells:europe,09169631.0[p].2011-8-17.
|
| [2] | 王颖,王雪,李波.两阶段细胞转化试验研究进展[j].中国新药杂志,2013,22(10):1133-1136.
|
| [3] | 王晨,张立实,何涛.染料木黄酮对nih/3t3细胞体外短期转化实验的影响[j].中国公共卫生,2002,16(11):48-49.
|
| [4] | 王李伟,仲伟鉴,应贤平,等.三羟异黄酮对balb/c-3t3细胞的转化作用[j].癌变·畸变·突变,2005,27(1):48-51.
|
| [5] | muramatsud,sasakik,kurodas,etal.comparisonofsensitivitytoarseniccompoundsbetweenabhas42celltransformationassayandabalb/c3t3celltransformationassay[j].mutatres,2009,675:66-70.
|
| [6] | sakaia,sasakik,muramatsud,etal.abhas42celltransformationassayon98chemicals:thecharacteristicsandperformanceforthepredictionofchemicalcarcinogenicity[j].mutatres,2010,702:100-122.
|
| [7] | yamakagek,suih,ohtar,etal.genotoxicpotentialandinvitrotumour-promotingpotentialof2-dodecylcyclobutanoneand2-tetradecylcyclobutanone,tworadiolyticproductsoffattyacids[j].mutatresgenettoxicolenvironmutagen,2014,770(1):95-104.
|
| [8] | weisenseed,potha,roemere,etal.cigarettesmoke-inducedmorphologicaltransformationofbhas42cellsinvitro[j].alternlabanim,2013,41(2):181-189.
|
| [9] | ohmorik,satoy,nakajimad,etal.characteristicsofthetransformationfrequencyatthetumorpromotionstageofairborneparticulateandgaseousmatterattensitesinjapan[j].environsciprocessimpacts,2013,15(5):1031-1040.
|
| [10] | benignir,bossac,tcheremenskaiao.invitrocelltransformationassaysforanintegrated,alternativeassessmentofcarcinogenicity:adata-basedanalysis[j].mutagenesis,2013,28(1):107-116.
|
| [11] | corvir,aardemam,gribaldol,etal.ecvamprevalidationstudyoninvitrocelltransformationassays:generaloutlineandconclusionsofthestudy[j].mutatres,2012,744:12-19.
|
| [12] | kimsh,kimcw,jeonsy,etal.chemopreventiveandchemotherapeuticeffectsofgenistein,asoyisoflavone,uponcancerdevelopmentandprogressioninpreclinicalanimalmodels[j].labanimres,2014,30(4):143-150.
|
| [13] | mahmoudam,yangw,boslandmc.soyisoflavonesandprostatecancer:areviewofmolecularmechanisms[j].jsteroidbiochemmolbiol,2014,140(1):116-132.
|
| [14] | 肖杨,刘然,刑丽娜,等.采用微团培养模型探讨染料木黄酮的发育毒性[j].癌变·畸变·突变,2010,22(4):271-275.
|
| [15] | 严继承,郑季彦,郑一凡,等.几种植物雌激素对细胞间隙连接通讯的影响[j].中华预防医学杂志,2005,2:55-57.
|
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