Adhesive layer is an essential part of tooth colored restorations which play an important role in decreasing the microleakage between the tooth and restoration material after polymerization shrinkage. The purpose of this study was to evaluate the effect of deferent adhesive system of bonding agent on microleakage of nanocomposite resin in class II cavities. Two different types of adhesive systems: universal adhesive (ExciTE) and a newly developed adhesive (Nano-Bond) and one type of light-cured resin restorative material (nanocomposite resin) were used in this study. These adhesives were applied to prepared tooth cavities by either manufactures’ instructions or by an experimental method (single or double application). Nanocomposite resin was then placed and light-cured for 40 seconds. Teeth were subjected to 500 thermal cycles between 5°C and 55°C and were immersed in 3% methylene blue solution for 24 hours; microleakage was observed microscopically. The data were analyzed by a two-way ANOVA. For comparison between groups, Tukey’s post-hoc test was used. Nanoparticles reinforced adhesive system shows low microleakage in compare with universal adhesive system, and application of two adhesive layers also can decrease the microleakage. The ability of stress absorption by adhesive layer after polymerization shrinkage of restoration material will reduce the microleakage.
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