Evaluation of Quantitative Phytochemicals, Liver Enzymes and Histological Changes in Isoniazid Induced Hepatotoxicity in Adult Male Wistar Rats Treated with Aqueous Extracts of Brysocarpus coccineus
Isoniazid induced
hepatotoxicity is a major concern in patients taking anti tuberculosis
treatment and prophylaxis. It can result in elevated serum liver enzymes and
hepatic failure. The aim of the study was to evaluate the phytochemicals and
ameliorative effects of aqueous extracts of Brysocarpus
coccineus on serum liver enzymes in isoniazid (INH) induced hepatotoxicity
in adult male Wistar rats. Thirty six (36) adult male Wistar rats were divided
into six groups of six rats each and were treated orally for 30 days as
follows: Group I: 1 ml/kg of distilled water; group II: Isoniazid (27 mg/kg);
group III: Isoniazid (27 mg/kg) + Livolin forte (20 mg/kg); group IV: Isoniazid
(27 mg/kg) + B. coccineus (200
mg/kg); group V: Isoniazid (27 mg/kg) + B.
coccineus (400 mg/kg); group VI:
Isoniazid (27 mg/kg) + B. coccineus (800
mg/kg). At the end of the experiments, the Wistar rats were sacrificed
and sera obtained for liver enzymes assay, whereas the liver tissue was also
harvested and used for histological studies. Tanins, saponins, alkaloids and flavonoids
were quantitatively present at 2.29%, 18.05%, 23.24% and 18.99%, respectively.
There was an increase in the serum AST and ALT in the isoniazid treated group,
which was reversed by livolin forte and the aqueous extracts at a dose of 200 mg/kg,
however the extracts increased the serum levels of AST and ALT at higher doses,
which was however not significant (p > 0.05) when compared to the controls. There was evidence of a reduction in
hepatocytes damage in the extract
treated groups when compared to the Isoniazid untreated group. In
conclusion, aqueous extracts of B. coccineus shows hepatoprotective effects at 200 mg/kg in isoniazid hepatotoxicity in
adult male Wistar rats.
References
[1]
Huang, Y.S. (2007) Genetic Polymorphisms of Drug-Metabolising Enzymes and the Susceptibility to Antituberculosis Drug-Induced Liver Injury. Expert Opinion on Drug Metabolism and Toxicology, 3, 1-8. https://doi.org/10.1517/17425255.3.1.1
[2]
Huang, Y.S. (2014) Review Article: Recent Progress in Genetic Variation and Risk of Antituberculosis Drug-Induced Liver Injury. Journal of the Chinese Medical Association, 77, 173.
[3]
Ching, S. (2011) A Study of Genetic Polymorphism Underlying Idiosyncratic Hepatotoxicity Due to Anti-Tuberculosis Medications. A thesis submitted in partial fulfilment of the requirements for the Degree of Doctor of Philosophy, Newcastle University, Newcastle, 23-34.
[4]
Gamaniel, K.S., Akah, P.A., Samson, A. and Wambebe, C.O. (1997) Evaluation of Nigerian Traditional Medicine: Effects of Gakani, a Herbal Anti-Asthmatic Drug. Journal of Ethnopharmacology, 55, 87 92. https://doi.org/10.1016/S0378-8741(96)01475-4
[5]
Wazis, C. H., Anuka, J.A., Timothy, S.Y., Zezi, A.U., Mohammed, G.T. and Hussaini, I.M. (2012) Acute Toxicity and in-Vivo Effects of Leaf Extracts of Byrsocarpus Coccineus Shum & Thonn in Pregnant Rat Uterus. Journal of Applied Pharmaceutical Science, 2, 130-136. https://doi.org/10.7324/JAPS.2012.21224
[6]
Dalziel, J.M. (1937) The Useful Plants of West Tropical Africa. A Crown Agent for Oversea Publication, London, 568-570.
[7]
Akindele, A.J. and Adeyemi, O.O. (2006) Evaluation of the Antidiarrhoeal Activity of Byrsocarpus coccineus. Journal of Ethnopharmacology, 108, 20-25. https://doi.org/10.1016/j.jep.2006.03.038
[8]
Amos, S., Binda, L., Kunle, O.F., Wambebe, C. and Gamaniel, K. (2002) Uterotonic Properties of the Ethanolic Extract of Byrsocarpus coccineus. Pharmaceutical Biology, 40, 33-38. https://doi.org/10.1076/phbi.40.1.33.5855
[9]
Laporta, J., Peters, T.L., Merriman, K.E., Vezina, C.M. and Hernandez, L.L (2013) Serotonin (5-HT) Affects Expression of Liver Metabolic Enzymes and Mammary Gland Glucose Transporters during the Transition from Pregnancy to Lactation. PLoS ONE, 8, 57847. https://doi.org/10.1371/journal.pone.0057847
[10]
Harbone, J.B. (1984) Phytochemical Methods. Capman and Hall, London, New York, Melbourne, Madras, 20-110. https://doi.org/10.1007/978-94-009-5570-7
[11]
Boham, B.A. and Kocipai-Abyazan, R. (1974) Flavonoids and Condensed Tannins from Leaves of Hawaiian Vaccinium vaticulatum and V. calycinium. Pacific Science, 48, 458-463.
[12]
Obdoni, B.O. and Ochuko, P.O. (2001) Phytochemical Studies and Comparative Efficacy of the Crude Extracts of Some Homostatic Plants in Edo and Delta States of Nigeria. Global Journal of Pure and Applied Science, 8, 203-208.
[13]
Chanwitheesuk, A., Teerawutgulrag, A. and Rakariyatham, N. (2005) Screening of Antioxidant Activity and Antioxidant Compounds of Some Edible Plants of Thailand. Food Chemistry, 92, 491-497.
[14]
Bergmeyer, H. and Walefeld, M. (1978) Kinetic Method for the Determination of TGO and TGP without Pyridoxal Phosphate. Clinica Chimica Acta, 24, 58.
[15]
Bowers, G.N.J. and Mc-Comb, R.B. (1966) A Continuous Spectrophotometric Method for Measurement the Activity of Serum Alkaline Phosphatase. Clinical Chemistry, 12, 73-78.
[16]
Hollman, P.C. and Arts, J.C. (2005) Polyphenols and Disease in Epidemiologic Studies. American Journal of Clinical Nutrition, 81, 317S-325S.
[17]
Manach, C., Scalbert, A., Morand, C., Remesy, C. and Jimenez (2004) Polyphenols: Food Sources and Bioavailability. American Journal of Clinical Nutrition, 79, 727-747.
[18]
Vaya, J. And Aviram, M. (2001) Nutritional Antioxidants: Mechanisms of Action, Analyses of Activities and Medical Applications. Current Medicinal Chemistry, 18, 99-117. https://doi.org/10.2174/1568013013359168
[19]
Duduku, K., Rosalam, S. and Awang, B. (2007) Phytochemical Antioxidants for Health and Medicine—A Move towards Nature. Biotechnology and Molecular Biology Review, 1, 97-104.
[20]
Benichou, C. (1990) Criteria of Drug-Induced Liver Disorders. Report of an International Consensus Meeting. Journal of Hepatology, 11, 272-276.
[21]
Olukiran, O.S., Akomolafe, R.O., Bamitale, K., Ajayi, A.O., Okonji, R.E. and Bejide, R.A. (2014) Protective and Curative Effects of Livolin Forte on Carbon Tetrachloride-Induced Liver Damage in Wistar Rats. Journal of Experimental and Integrative Medicine, 4, 57-65. https://doi.org/10.5455/jeim.310813.or.088
[22]
Adams, D.H., Ju, C., Ramaiah, S.K., Uetrecht, J. and Jaeschke, H. (2010) Mechanisms of Immune-Mediated Liver Injury. Toxicological Sciences, 115, 307-321. https://doi.org/10.1093/toxsci/kfq009