Combinatorial chemistry has been a focus of research activity in modern drug discovery and biotechnology. It is a concept by which a vast library of molecular diversity is synthesized and screened for target properties. This report is to illustrate the application of enzyme technology using the concept of combinatorial chemistry as a novel approach for the bioconversion of plant fibers. Citrus pectin was subjected to combinatorial enzyme digestion to create libraries of pectic oligosaccharides with diverse structural variants. Repeated cycles of fractionation and screening resulted in the isolation and identification of an active oligoGalA species with antimicrobial activity.
References
[1]
Kennedy, J.P., Williams, L., Bridges, T.M., Daniels, R.N., Weaver, D. and Lindsley, G.W. (2008) Application of Combinatorial Chemistry Science on Modern Drug Discovery. Journal of Combinatorial Chemistry, 10, 345-354. https://doi.org/10.1021/cc700187t
[2]
Seneca, P., Fassina, G., Frecer, V. and Miertus, S. (2014) The Effects of Combinatorial Chemistry and Technologies on Drug Discovery and Biotechnology—A Mini Review. Nova Biotechnologica et Chimica, 13, 87-108. https://doi.org/10.1515/nbec-2015-0001
[3]
Lindell, S.D., Pattenden, L.C. and Shannon, J. (2009) Combinatorial Chemistry in the Agrosciences. Bioorganic & Medicinal Chemistry, 17, 4035-4046. https://doi.org/10.1016/j.bmc.2009.03.027
[4]
Wong, D.W.S. and Robertson, G. (2004) Applying Combinatorial Chemistry and Biology to Food Research. Journal of Agricultural and Food Chemistry, 52, 7187-7198. https://doi.org/10.1021/jf040140i
[5]
Wong, D.W.S. and Robertson, G. (1999) Combinatorial Chemistry and Its Applications in Agriculture and Food. In: Shahidi et al., Eds., Chemicals via Higher Plant Bioengineering, Kluwer Academic/Plenum Publishers, New York, 91-105. https://doi.org/10.1007/978-1-4615-4729-7_8
[6]
Herrmann, A. (2014) Dynamic Combinatorial/Covalent Chemistry: A Tool to Read, Generate and Modulate the Bioactivity of Compounds and Compound Mixtures. Chemical Society Reviews, 43, 1899-1933. https://doi.org/10.1039/C3CS60336A
[7]
Miller, G.L. (1959) Use of Dinitrosalicyclic Acid Reagent for Determination of Reducing Sugar. Analytical Chemistry, 31, 426-428. https://doi.org/10.1021/ac60147a030
[8]
Masuko, T., Minami, A., Iwasaki, N., Majima, T., Nishimura, S.-I. and Lee, Y.C. (2005) Carbohydrate Analysis by a Phenol-Sulfuric Acid Method in Microplate Format. Analytical Biochemistry, 239, 69-72. https://doi.org/10.1016/j.ab.2004.12.001
[9]
Andrews, J.M. (2001) Determination of Minimum Inhibitory Concentrations. Journal of Antimicrobial Chemotherapy, 48, 5-16. https://doi.org/10.1093/jac/48.suppl_1.5
[10]
Nychas, G.J.E. (1995) Natural Antimicrobials from Plants. In: Gould, G.W., Ed., New Methods of Foods Preservation, Blackie Academic Chapman & Hall, Glasgow, 53-83, 3-2, 87-108. https://doi.org/10.1007/978-1-4615-2105-1_4
[11]
Garrote, G., Cruz, J.M., Moure, A., Dominguez, H. and Parajo, J.C. (2004) Antioxidant Activity of Byproducts from the Hydrolytic Processing of Selected Lignocellulosic Materials. Trends in Food Science & Technology, 15, 191-200. https://doi.org/10.1016/j.tifs.2003.09.016
[12]
Aziz, N.H., Farag, S.E., Mousa, L.A. and Abo-Zaid, M.A. (1998) Comparative Antibacterial and Antifungal Effects of Some Phenolic Compounds. Microbios, 93, 43-54.
[13]
Christakopoulos, P., Katapodis, P., Kalogeris, E., Kekos, D., Macris, B.J., Stamatis, H. and Shaltsa, H. (2003) Antimicrobial Activity of Acidic Xylo-Oligosaccharides Produced by Family 10 and 11 Endoxylanases. International Journal of Biological Macromolecules, 31, 171-175. https://doi.org/10.1016/S0141-8130(02)00079-X
[14]
Chaan, F., Belghith-Ferrdri, L., Zaouri-Ellouzi, S., Driss, D., Bilbech, M., Kallel, F., Bouaziz, F., Mehdi, Y., Ellouz-Chaabouni, S. and Ghorbel, R. (2016) Antibacterial and Antioxidant Properties of Mixed Linkage Beta-Oligosaccharides from Extracted β-Glucan Hydrolyzed by Penicillium occitanis EGL Lichenase. Natural Product Research, 30, 1353-1359. https://doi.org/10.1080/14786419.2015.1056185
[15]
Khan, S., Tendervik, A., Sletta, H., Klinkenberg, G., Emanuel, C., Onseyen, E., Myrvold, R., Howe, R.A., Walsh, T.R., Hill, K.E. and Thomas, D.W. (2012) Overcoming Drug Resistance with Alginate Oligosaccharides Able to Potentiate the Action of Selected Antibiotics. Antimicrobial Agents Chemotherapy, 56, 5134-5141. https://doi.org/10.1128/AAC.00525-12
[16]
Tendervik, A., Sletta, H., Klinkenberg, G., Emanuel, C., Powell, L.C., Pritchard, M.F., Khan, S., Craine, K.M., Onseyen, E., Rye, P.D., Wright, C., Thomas, D.W. and Hill, K.E. (2014) Alginate Oligosaccharides Inhibit Fungal Cell Growth and Potentiate the Activity of Antifungals against Candida and Aspergillus spp. PLOS One, 9, e112518. https://doi.org/10.1371/journal.pone.0112518
[17]
Huyghebaert, G., Ducatelle, R. and Van Immerseel, F. (2011) An Update on Alternatives to Antimicrobial Growth Promoters for Broilers. The Veterinary Journal, 187, 182-188. https://doi.org/10.1016/j.tvjl.2010.03.003
[18]
Mussatto, S.I. and Mancilha, I.M. (2007) Non-Digestible Oligosaccharides: A Review. Carbohydrate Polymers, 68, 587-597. https://doi.org/10.1016/j.carbpol.2006.12.011
