Bone Marrow Cells Obtained from Old Animals Differ from the Young Animals Cells in Their Ability to Divide and in Response to the Presence of Liver Fibrosis in Primary Culture
Dynamics
of bone marrow cells number (BMC) in the primary culture isolated from young (3
months) and old (20 months) Wistar rats was investigated.Proliferative activity of BMC of old animals was
2 times higher than that of young animals in the primary culture. Such
superiority of the proliferative activity of BMC in the primary culture
obtained from old animals is associated with the ability to actively divide
lymphocytes and longer “lifespan” of segmented neutrophils obtained
from old animals. It should be noted, that the lymphocytes of young animals did
not proliferate in the primary culture. The content of intracellular calcium in
BMC in the cells of old animals was 3 times higher compared with cells of young
animals, which revealed the relationship of intracellular calcium and
proliferative activity of BMC. Induction of liver fibrosis led to an increase
in the lymphocyte content in young animals by 167%, and in old ones only by
26%, while the lymphocytes of young animals acquired the ability to proliferate
in the primary culture. It has been suggested that such differences in the
behavior of BMC in primary culture obtained in young and old animals reflect
differences in the BMC microenvironment of young and old animals, which leads
to changes in the epigenetic-metabolic characteristics of BMC.
References
[1]
Ideker, T., Galitski, T. and Hood, L. (2001) A New Approach to Decoding Life: Systems Biology. Annual Review of Genomics and Human Genetics, 2, 343-372. https://doi.org/10.1146/annurev.genom.2.1.343
[2]
Krause, D.S., Fulzele, K., Catic, A., Sun, C.C., Dombkowski, D., Hurley, M.P., Divieti-Pajevic, P., et al. (2013) Differential Regulation of Myeloid Leukemias by the Bone Marrow Microenvironment. Nature medicine, 19, 1513-1517. https://doi.org/10.1038/nm.3364
[3]
Johnson, R. and Halder, G. (2014) The Two Faces of Hippo: Targeting the Hippo Pathway for Regenerative Medicine and Cancer Treatment. Nature Reviews Drug Discovery, 13, 63-79. https://doi.org/10.1038/nrd4161
[4]
Atala, A., Lanza, R., Mikos, T. and Nerem, R. (2018) Principles of Regenerative Medicine. Academic Press, Cambridge.
[5]
Cooper, S. (2006) Distinguishing between Linear and Exponential Cell Growth during the Division Cycle: Single-Cell Studies, Cell-Culture Studies, and the Object of Cell-Cycle Research. Theoretical Biology and Medical Modelling, 3, 10. https://doi.org/10.1186/1742-4682-3-10
[6]
Orlic D., Kajstura J., Chimenti S., et al. (2000) Bone Marrow Cells Regenerate Infarcted Myocardium. Nature, 410, 701-705. https://doi.org/10.1038/35070587
[7]
Dong, L., Yu, W.M., Zheng, H., Loh, M.L., Bunting, S.T., Pauly, M., Qu, C K., et al. (2016) Leukaemogenic Effects of Ptpn11 Activating Mutations in the Stem Cell Microenvironment. Nature, 539, 304-308. https://doi.org/10.1038/nature20131
[8]
Kumar, V., Patel, S., Tcyganov, E. and Gabrilovich, D.I. (2016) The Nature of Myeloid-Derived Suppressor Cells in the Tumor Microenvironment. Trends in immunology, 37, 208-220. https://doi.org/10.1016/j.it.2016.01.004
[9]
Gao, F., Chiu, S.M., Motan, D.A.L., Zhang, Z., Chen, L., Ji, H.L., Lian, Q., et al. (2017) Mesenchymal Stem Cells and Immunomodulation: Current Status and Future Prospects. Cell Death & Disease, 7, e2062. https://doi.org/10.1038/cddis.2015.327
Bozhkov, A.I., Klimova, E.M., Nikitchenko, Y.V., Davydov, V.V., Zvyagintseva, O.V., Kurguzova, N.I., Naglov, A.V., et al. (2014) Stem Cells Take Part in Regulation of Prooxidant Activity and Immunity at Liver Fibrosis. American Journal of Biomedical and Life Sciences, 2, 5-12. https://doi.org/10.11648/j.ajbls.s.2014020601.12
[12]
Goodell, M.A. and Rando, T.A. (2015) Stem Cells and Healthy Aging. Science, 350, 1199-1204. https://doi.org/10.1126/science.aab3388
[13]
Kusumbe, A.P., Ramasamy, S.K., Itkin, T., Mäe, M.A., Langen, U.H., Betsholtz, C., Adams, R.H., et al. (2016) Age-Dependent Modulation of Vascular Niches for Haematopoietic Stem Cells. Nature, 532, 380-384. https://doi.org/10.1038/nature17638
[14]
Fafián-Labora, J., Fernández-Pernas, P., Fuentes, I., De Toro, J., Oreiro, N., Sangiao-Alvarellos, S., Arufe, M.C., et al. (2015) Influence of Age on Rat Bone-Marrow Mesenchymal Stem Cells Potential. Scientific Reports, 5, Article No. 16765. https://doi.org/10.1038/srep16765
[15]
Sardo, V.L., Ferguson, W., Erikson, G.A., Topol, E.J., Baldwin, K.K. and Torkamani, A. (2017) Influence of Donor Age on Induced Pluripotent Stem Cells. Nature Biotechnology, 35, 69-74. https://doi.org/10.1038/nbt.3749
[16]
Lv, F.J., Tuan, R.S., Cheung, K.M. and Leung, V.Y. (2014) Concise Review: The Surface Markers and Identity of Human Mesenchymal Stem Cells. Stem Cells, 32, 1408-1419. https://doi.org/10.1002/stem.1681
[17]
Guo, Y., Liu, L., Sun, M., Hai, Y., Li, Z. and He, Z. (2015) Expansion and Long-Term Culture of Human Spermatogonial Stem Cells via the Activation of SMAD3 and AKT Pathways. Experimental Biology and Medicine, 240, 1112-1122. https://doi.org/10.1177/1535370215590822
[18]
(1986) Council Directive 86/609/EEC of 24 November 1986 on the Approximation of Laws, Regulations and Administrative Provisions of the Member States Regarding the Protection of Animals Used for Experimental and Other Scientific Purposes. Official Journal L, 358, 0001-0028.
[19]
Bozhkov, A.I., Nikitchenko, Y.V., Klimova, E.M., Linkevych, O.S., Lebid, K.M., Al-Bahadli, A.M.M. and Alsardia, M.M.A. (2017) Young and Old Rats Have Different Strategies of Metabolic Adaptation to Cu-Induced Liver Fibrosis. Advances in Gerontology, 7, 41-50. https://doi.org/10.1134/S2079057017010040
[20]
Amend, S.R., Valkenburg, K.C. and Pienta, K.J. (2016) Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation. Journal of Visualized Experiments, No. 110. https://doi.org/10.3791/53936
[21]
Bozhkov, А.I., Kabachnyy, V.I., Bondar, А.Y., Chumak, I.V. and Kolot, N.V. (2014) Hormesis Effect and the Influence of Ultra-Low Glycosides Doses on the BMC Proliferative Activity in Culture. Journal of Harmonized Research in Pharmacy, 3, 154-166.
[22]
Lewandowski, K., Kowalik, M.M., Pawlaczyk, R., Rogowski, J. and Hellmann, A. (2012) Microscopic Examination of Bone Marrow Aspirate in Healthy Adults-Comparison of Two Techniques of Slide Preparation. International Journal of Laboratory Hematology, 34, 254-261. https://doi.org/10.1111/j.1751-553X.2011.01387.x
[23]
Merritt, J.E., McCarthy, S.A., Davies, M.P. and Moores, K.E. (1990) Use of Fluo-3 to Measure Cytosolic Ca2+ in Platelets and Neutrophils. Loading Cells with the Dye, Calibration of Traces, Measurements in the Presence of Plasma, and Buffering of Cytosolic Ca2+. Biochemical Journal, 269, 513-519. https://doi.org/10.1042/bj2690513
[24]
Chen, C., Tang, Z., Song, Q., Yang, M., Shi, Q. and Weng, Y. (2016) Downregulated microRNA-23b Promotes BMP9-Mediated Osteogenesis in C2C12 Myoblast Cells by Targeting Runx2. Molecular Medicine Reports, 13, 2492-2498. https://doi.org/10.3892/mmr.2016.4814
[25]
Quah, B.J., Warren, H.S. and Parish, C.R. (2007) Monitoring Lymphocyte Proliferation in Vitro and in Vivo with the Intracellular Fluorescent Dye Carboxy Fluorescein Diacetate Succinimidyl Ester. Nature Protocols, 2, 2049. https://doi.org/10.1038/nprot.2007.296
[26]
Carvalho, L.O., Aquino, E.N., Neves, A.C.D. and Fontes, W. (2015) The Neutrophil Nucleus and Its Role in Neutrophilic Function. Journal of Cellular Biochemistry, 116, 1831-1836. https://doi.org/10.1002/jcb.25124
[27]
Görlach, A., Bertram, K., Hudecova, S. and Krizanova, O. (2015) Calcium and ROS: A Mutual Interplay. Redox Biology, 6, 260-271. https://doi.org/10.1016/j.redox.2015.08.010
[28]
Bozhkov, A.I., Ivanov, E.G., Kuznetsova, Y.A., Ohiienko, S.L. and Bondar, A.Y. (2017) Copper-Induced Liver Fibrosis Affects the Behavior of BMC in Primary Culture. Frontiers in Biology, 12, 271-279. https://doi.org/10.1007/s11515-017-1458-y
[29]
Bozhkov, A.I., Ohiienko, S.L., Kuznetsova, Y.A., Bondar, A.Y., Marchenko, V.P. and Gumennaya, M.S. (2017) Donor Age Affects Behavior and Sensibility of BMC to Copper Ions in Primary Culture. Advances in Gerontology, 7, 336-344. https://doi.org/10.1134/S2079057017040026
[30]
Morrison, S.J. and Scadden, D.T. (2014) The Bone Marrow Niche for Haematopoietic Stem Cells. Nature, 505, 327. https://doi.org/10.1038/nature12984
[31]
Crane, G.M., Jeffery, E. and Morrison, S.J. (2017) Adult Haematopoietic Stem Cell Niches. Nature Reviews Immunology, 17, 573. https://doi.org/10.1038/nri.2017.53
[32]
Vazquez, M.I., Catalan-Dibene, J. and Zlotnik, A. (2015) B Cells Responses and Cytokine Production Are Regulated by Their Immune Microenvironment. Cytokine, 74, 318-326. https://doi.org/10.1016/j.cyto.2015.02.007
[33]
Squillaro, T., Peluso, G. and Galderisi, U. (2016) Clinical Trials with Mesenchymal Stem Cells: An Update. Cell Transplantation, 25, 829-848. https://doi.org/10.3727/096368915X689622
[34]
Nathan, C. (2006) Neutrophils and Immunity: Challenges and Opportunities. Nature Reviews Immunology, 6, 173. https://doi.org/10.1038/nri1785
[35]
Pornsiriwong, W., Estavillo, G.M., Chan, K.X., Tee, E.E., Ganguly, D., Crisp, P.A., Yong, M.T., et al. (2017) A Chloroplast Retrograde Signal, 3’-Phosphoadenosine 5’-Phosphate, Acts as a Secondary Messenger in Abscisic Acid Signaling in Stomatal Closure and Germination. Elife, 6, e23361. https://doi.org/10.7554/eLife.23361
[36]
Prati, C. and Gandolfi, M.G. (2015) Calcium Silicate Bioactive Cements: Biological Perspectives and Clinical Applications. Dental Materials, 31, 351-370. https://doi.org/10.1016/j.dental.2015.01.004
