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- 2018
铁载体在假交替单胞菌Cd2+去除中的作用
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Abstract:
摘要： 为探索铁载体在微生物重金属去除过程中的作用,利用一株高效除镉菌——假交替单胞菌Pseudoaltermonas species. SCSE709-6(P. sp. SCSE709-6),研究镉(Cd2+)的添加对铁载体产量的影响以及铁载体的添加对菌体去除Cd2+的影响。结果表明:菌体代谢能够产生羟基羧酸盐型铁载体,其产量与细菌生物量相关。在Cd2+浓度为0~0.4 mmol/L时铁载体量呈先增后减的变化趋势,当为0.2 mmol/L时铁载体量最大。当向含镉培养液中加入铁载体时,细菌的适应期缩短,说明铁载体能够与Cd2+络合,从而降低镉的生物毒性,提高细菌对镉的去除效率。研究结果为P. sp. SCSE709-6高效除镉提供了一种科学解释,在微生物修复重金属镉污染中具有潜在的实际应用价值。
Abstract: In order to explore the role of siderophore in the process of removal of heavy metals, Pseudoaltermonas sp. SCSE709-6(P. sp. SCSE709-6), was selected as a representative bacterium. The effect of Cd2+ addition on siderophore production by P. sp. SCSE709-6 and the effect of siderophore addition on the removal of Cd2+ were studied, respectively. Upon O-CAS assay, P. sp. SCSE709-6 showed a vivid positive result for siderophore production. Assays based on chemical properties indicated that siderophore produced by P. sp. SCSE709-6 was carboxylate type. The siderophore yield was correlated with the biomass. Siderophore production was increased first and then decreased when the Cd2+ concentration was 0~0.4 mmol/L, notable this value reached maximum at 0.2 mmol/L. It accelerated the adaptation of P. sp. SCSE709-6 to Cd2+ while siderophore was added to the culture medium, as siderophore could be combined with Cd2+ to reduce the toxicity of cadmium, leading to high removal efficiency of Cd2+. The results provided a scientific explanation of why P. sp. SCSE709-6 is highly efficient in removal of cadmium and P. sp. SCSE709-6 could be recommended as a potential candidate for application in bioremediation of heavy metals
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