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-  2019 

miR-148a通过靶定DNMT1调节大鼠间充质干细胞向心肌细胞的分化 miR-148a regulates cardiomyocyte differentiation of mesenchymal stem cells via targeting DNMT1

Keywords: miR-148a,DNMT1,间充质干细胞,分化,心肌细胞

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Abstract:

目的:探讨miR-148a对间充质干细胞(MSCs)向心肌细胞分化的调控作用。方法:将miR-148a及对照分别转染入大鼠骨髓间充质干细胞(BMSCs),与新生大鼠心室心肌细胞(NRVMs)共培养,观察BMSCs的分化,通过增强及抑制miR-148a的表达水平观察miR-148a对BMSCs向心肌细胞分化的调控作用。运用荧光素酶报告实验、实时荧光定量聚合酶链反应(qRT-PCR)和免疫印迹(Western Blot)验证miR-148a靶向下调DNA甲基转移酶1(DNMT1)参与调节该过程。结果:过表达miR-148a有利于BMSCs向心肌细胞的分化,而当利用抑制剂下调miR-148a的表达,则阻止BMSCs向心肌细胞的分化。荧光素酶报告实验、qRT-PCR和Western Blot实验发现miR-148a可以靶向下调DNMT1的表达,且DNMT1参与调节上述过程。结论:miR-148a通过靶向下调DNMT1促进MSCs向心肌细胞的分化过程

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