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花生芽中白藜芦醇的微波和超声波组合提取研究
Extraction of Resveratrol from Peanut Bud with Combining Microwave Irradiation and Ultrasonic Wave

DOI: 10.12677/MC.2018.22008, PP. 50-55

Keywords: 花生芽,白藜芦醇,微波提取,超声波提取,正交试验,HPLC
Peanut Bud
, Resveratrol, Extraction with Microwave Irradiation, Ultrasonic Waves Extraction, Orthogonal Test, HPLC

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Abstract:

本文采用微波和超声波组合法从花生芽中提取白藜芦醇浸膏,并以HPLC法测定所得到的浸膏中白藜芦醇含量。在恒定微波功率600 W和超声波功率1500 W基础上,通过L16 (43)正交试验,考察了乙醇浓度、作用时间和提取温度三个因素对提取效果的影响。优选花生芽在微波和超声波组合作用条件下的最佳提取工艺,并考察该提取法提取白藜芦醇的效果。研究结果表明,微波和超声波组合作用对于花生芽中提取白藜芦醇具有较高的效率。采用这种组合作用的提取方法,乙醇浓度为主要影响因素,然后是提取温度,最后是作用时间。最优的提取工艺是提取溶剂为40%乙醇、微波和超声波作用时间为3 min和提取温度为65℃。
In this paper, Resveratrol plant extract was extracted from Peanut buds by the combination ex-traction method of microwave irradiation and ultrasonic wave, and the content of resveratrol was determined by HPLC. Based on the constant microwave power of 600 W and ultrasonic wave power of 1500 W, the main and secondary influencing factors of ethanol concentration, action time and extraction temperature were investigated through the orthogonal test of L16 (43). The results show that the combination of microwave irradiation and ultrasonic wave has high efficiency in extracting resveratrol from peanut buds. With the extraction method combined microwave irradiation and ultrasonic wave, the ethanol concentration is the main influencing factor, then the extraction temperature, and finally the action time. The optimal extraction process is 40% ethanol of the extract solvent, 3 min of the action time, and 65?C of the extracting temperature.

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