人类基因序列二维可视化分析
Two-Dimensional Visualization Analysis of Homo Sapiens Gene Sequences

DOI: 10.12677/HJCB.2019.93009, PP. 59-80

Keywords: 染色体序列，mRNA，ncRNA，cDNA，CDS，可视化，概率测量, mRNA, ncRNA, cDNA, CDS, Visualization, Probability Measurement

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Abstract:

染色体是遗传信息载体，正常人的体细胞染色体数目为23对，并有一定的形态和结构。人类从猿到人的长期进化过程中丢失了数万个基因，研究表明，基因中缺失的部分能够反映人类在进化史中的选择，科学家认为DNA的丢失和复制现象可能会是重要的进化动力，研究这些基因变化具有重要意义。人类Y染色体缺失数量较为明显，数百万年前，Y染色体上的基因还有大约1500个之多，而现在却总共只剩下40个。基因缺失属于基因突变的一种，基因突变可以发生在发育的任何时期，在人类中因基因突变引发的疾病有多种，而生物性状的表达由基因控制，从基因序列表达到蛋白质过程中涉及相关的序列有mRNA，ncRNA、cDNA、CDS。本文致力于探索这几种序列之间的关系，以可视化形式将这些基因序列进行可视化。在本文中使用变值概率统计图示表示方法对人类较为特殊的2号染色体、Y染色体、mRNA、CDS、cDNA和ncRNA序列进行分段概率测量，映射成多个2D概率统计图，从而对不同的基因序列特征分布进行比较分析。
Chromosomes are the carriers of genetic information. The number of somatic chromosomes in normal human is 23 pairs, and they have certain morphology and structure. Human beings have lost tens of thousands of genes in the long-term evolution process from apes to humans. Studies have shown that the missing parts of genes can reflect human selection in the evolutionary history. Scientists believe that the loss and replication of DNA may be an important evolutionary motive force. It is of great significance to study these gene changes. The number of Y chromosome deletions in humans is obvious. Millions of years ago, there were about 1500 genes on Y chromosome, but now there are only 40. Gene deletion is a kind of gene mutation. Gene mutation can occur at any stage of development. There are many diseases caused by gene mutation in human. Gene controls the expression of biological traits. Gene sequence from expression to protein is related to the sequence of RNA, ncRNA, cDNA, CDS. This paper is devoted to exploring the relationship between these sequences and visualizing these gene sequences in a visual form. In this paper, the variable probability statistical graphical representation method is used to measure the fragmentation probability of the special sequences of chromosome 2, Y, mRNA, CDS, cDNA and ncRNA in Homo sapiens and map them into several 2D probability statistical maps, so as to compare and analyze the distribution of different gene sequences.

References

[1]	刘笑麟. 人类进化, 基因大流失[J]. 大科技: 科学之谜(A), 2018(4): 46-47.
[2]	晏子悠. 谁偷了我的染色体[J]. 大科技(科学之谜), 2007(2): 42-43.
[3]	http://europepmc.org/articles/PMC4987893
[4]	潘书贤, 周光明, 胡文涛. 非编码RNA参与昼夜节律调控研究进展[J]. 航天医学与医学工程, 2019, 32(2): 178-182.
[5]	杜娟, 陈亚妮, 史海燕, 吴博, 王爱红, 殷松娜. 长链非编码RNA LINC00519在胃癌中的表达及其临床意义[J/OL]. 山西医科大学学报, 2019(4): 484-488.
[6]	Li, W., Wang, R., Ma, J.Y., et al. (2017) A Human Long Non-Coding RNA ALT1 Controls the Cell Cycle of Vascular Endothelial Cells via ACE2 and Cyclin D1 Pathway. International Journal of Experimental Cellular Physiology Biochemistry & Pharmacology, 43, 1152-1167. https://doi.org/10.1159/000481756
[7]	解小莉. 生物序列的分析方法及其进化模型研究[D]: [博士学位论文]. 杨凌: 西北农林科技大学, 2012.
[8]	张柱金. DNA序列二维可视化研究[D]: [博士学位论文]. 武汉: 华中科技大学, 2011.
[9]	Zheng, J. (2018). Variant Construction from Theoretical Foundation to Applications. Springer, Ber-lin.
[10]	郑智捷. 在变值测量模拟中的条件概率统计分布[J]. 光子学报, 2011, 40(11): 1662-1666.
[11]	完竹, 郑智捷. DNA序列一维分段测量分布可视化[J]. 云南大学学报(自然科学版), 2013, 35(S2): 1-6.
[12]	吉艳. 基于变值测量的心电数据序列可视化应用研究[D]: [硕士学位论文]. 昆明: 云南大学, 2016.
[13]	刘玉倩, 郑智捷. 编码和非编码DNA序列的可视化分析[J]. 计算生物学, 2014, 4(2): 20-31.
[14]	Mao, Y., Zheng, J. and Liu, W. (2017) Mapping Whole DNA Sequence on Variant Maps. Proceedings of the 2017 IEEE/ACM International Conference on Advances in Social Networks Analysis and Mining, Sydney, 31 July-3 August 2017, 1037-1040. https://doi.org/10.1145/3110025.3110140
[15]	刘文嘉, 郑智捷. DNA序列互补匹配特征分析及可视化系统[J]. 计算生物学, 2015, 5(4): 49-57.
[16]	王树林, 王戟, 陈火旺, 张波云. 基于分形的DNA序列可视化表示研究[J]. 计算机科学, 2006(7): 158-163.
[17]	王安慧. 基因组信息的计算机可视化若干关键技术研究[D]: [博士学位论文]. 沈阳: 东北大学, 2010.
[18]	封海清, 陆祖宏. 一种新型的基于图像的DNA序列可视化模型[J]. 生物信息学, 2014, 12(2): 133-139.
[19]	Djebali, S., et al. (2012) Landscape of Transcription in Human Cells. Nature, 489, 101-108.
[20]	李平, 庞智, 朱剑云, 孙琛琛, 孙康云. 非编码RNA对冠心病发生发展影响的研究进展[J/OL]. 医学综述, 2019(8): 1474-1479. http://kns.cnki.net/kcms/detail/11.3553.r.20190419.1033.008.html, 2019-05-05.
[21]	崔薇, 陈楠, 苗震, 李和平, 刘伟石, 夏彦玲. 梅花鹿PTN基因cDNA克隆及表达分析[J]. 黑龙江畜牧兽医, 2019(7): 129-132.
[22]	孙盛明, 傅洪拓, 宣富君, 戈贤平, 朱健, 吴旭干. 青虾C型凝集素结构域家族3的cDNA克隆、原核表达和定位[J/OL]. 水产学报, 1-14. http://kns.cnki.net/kcms/detail/31.1283.s.20190408.0929.012.html, 2019-04-30.
[23]	何鹏, 江世贵, 李运东, 杨其彬, 姜松, 杨丽诗, 黄建华, 周发林. 斑节对虾GLUT1基因cDNA的克隆与表达分析[J]. 南方水产科学, 2019, 15(2): 72-82.

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