Human African trypanosomiasis (HAT) affects up to
half a million people every year in sub-Saharan Africa. Interruption of
transmission of the disease by early diagnosis and treatment is core to the
control and eventual elimination of HAT. The routine diagnostic method for HAT
is light microscopy of blood samples. The present study sought to evaluate the
potential of TbgI2 and TbgI17 tandem repeat antigens as
candidates for the diagnosis of Trypanosoma
bruceirhodesiense. The expressed proteins were purified and the
antigenic reactivity evaluation was done using multiplex assay using sera obtained from HAT patients.
Receiver operating characteristic analysis showed that recombinant antigen,
TbgI2 had high sensitivity for sera from patients infected with T. b. rhodesiense with the
area under the curve being 0.577 and a sensitivity of 0.641 and specificity 0.650.
The results suggest that TbgI2 is a potential biomarker for T. b. rhodesiense HAT
serodiagnostic tests.
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