Innate Cytokine Responses and Toll-Like Receptor Induced by Recombinant Porcine Rotavirus VP6 and VP7 Proteins Expressing in Lactobacillus plantarum NC8 Strain Colonization in Mice
The significant function of Toll-like receptors (TLR) is the detection of microbes by host guard cells that guide to the innate immune responses and to the successive adaptive. The current study patterns of TLR2, TLR3 and TLR9 expressing antigen presenting cells (APCs) in blood of mice after colonization with L. plantarum NC8 strain were assessed. The power of L. plantarum on serum innate cytokine and TLR responses stimulated by recombinant NC8-pSIP409-pgsA-VP6-DCpep, NC8-pSIP409-pgsA-VP7-DCpep and NC8-pSIP409-pgsA were also assessed. We confirmed that L. plantarum NC8 stimulated powerful TLR2 expressing APC responses in blood Recombinant strain stimulated a TLR3 response in spleen, and TLR9 responses were stimulated in blood or in spleen. Recombinant NC8-pSIP409-pgsA-VP6-DCpep, NC8-pSIP409-pgsA-VP7-DCpep on TLR2 and TLR9 expressing APC responses has a preservative outcome, reliable with the DCpep adjuvant outcome. In serum the recombinant NC8-pSIP409-pgsA-VP6-DCpep, NC8-pSIP409-pgsA-VP7-DCpep has increased the IL-4 and IFN-γ responses, except that on the TLR3 and TLR9 expressing CD14 APC responses it had an oppressive consequence in spleen and the IFN-α response in serum-stimulated by PRV. Our results give details that following PRV infection after immunization with NC8-pSIP409-pgsA-VP6-DCpep, NC8-pSIP409-pgsA-VP7-DCpep, the systemic TLR2, TLR3, and TLR9 expressing cDC and macrophage/monocyte responses.
References
[1]
Wen, K., Azevedo, M.S., Gonzalez, A., Zhang, W., Saif, L.J., Li, G., Yousef, A. and Yuan, L. (2009) Toll-Like Receptor and Innate Cytokine Responses Induced by Lactobacilli Colonization and Human Rotavirus Infection in Gnotobiotic Pigs. Veterinary Immunology and Immunopathology, 127, 304-315. https://doi.org/10.1016/j.vetimm.2008.10.322
[2]
Kaisho, T. and Akira, S. (2006) Toll-Like Receptor Function and Signaling. Journal of Allergy and Clinical Immunology, 117, 979-987. https://doi.org/10.1016/j.jaci.2006.02.023
[3]
Akira, S. and Hemmi, H. (2003) Recognition of Pathogen-Associated Molecular Patterns by TLR Family. Immunology Letters, 85, 85-95. https://doi.org/10.1016/S0165-2478(02)00228-6
[4]
Uematsu, S. and Akira, S. (2006) Toll-Like Receptors and Innate Immunity. Journal of Molecular Medicine, 84, 712-725. https://doi.org/10.1007/s00109-006-0084-y
[5]
Seth, R.B., Sun, L., Ea, C.K. and Chen, Z.J. (2005) Identification and Characterization of MAVS, a Mitochondrial Antiviral Signaling Protein that Activates NF-κB and IRF 3. Cell, 122, 669-682. https://doi.org/10.1016/j.cell.2005.08.012
[6]
Sandor, F. and Buc, M. (2005) Toll-Like Receptors. II. Distribution and Pathways Involved in TLR Signalling. Folia Biologica, 51, 188-197.
[7]
Hug, H., Mohajeri, M.H. and La Fata, G. (2018) Toll-Like Receptors: Regulators of the Immune Response in the Human Gut. Nutrients, 10, 203. https://doi.org/10.3390/nu10020203
[8]
Sieling, P.A. and Modlin, R.L. (2002) Toll-Like Receptors: Mammalian “Taste Receptors” for a Smorgasbord of Microbial Invaders. Current Opinion in Microbiology, 5, 70-75. https://doi.org/10.1016/S1369-5274(02)00288-6
[9]
Parashar, U.D., Gibson, C.J., Bresee, J.S. and Glass, R.I. (2006) Rotavirus and Severe Childhood Diarrhea. Emerging Infectious Diseases, 12, 304-306. https://doi.org/10.3201/eid1202.050006
[10]
Shonyela, S.M., Wang, G., Yang, W., Yang, G. and Wang, C. (2017) New Progress regarding the Use of Lactic Acid Bacteria as Live Delivery Vectors, Treatment of Diseases and Induction of Immune Responses in Different Host Species Focusing on Lactobacillus Species. World Journal of Vaccines, 7, 43-75. https://doi.org/10.4236/wjv.2017.74004
[11]
Ljungh, A. and Wadstrom, T. (2006) Lactic Acid Bacteria as Probiotics. Current Issues in Intestinal Microbiology, 7, 73-89.
[12]
Herias, M.V., Hessle, C., Telemo, E., Midtvedt, T., Hanson, L.A. and Wold, A.E. (1999) Immunomodulatory Effects of Lactobacillus plantarum Colonizing the Intestine of Gnotobiotic Rats. Clinical and Experimental Immunology, 116, 283-290. https://doi.org/10.1046/j.1365-2249.1999.00891.x
[13]
Yasui, H., Shida, K., Matsuzaki, T. and Yokokura, T. (1999) Immunomodulatory Function of Lactic Acid Bacteria. Antonie van Leeuwenhoek, 76, 383-389. https://doi.org/10.1023/A:1002041616085
[14]
Zocco, M.A., dal Verme, L.Z., Cremonini, F., Piscaglia, A.C., Nista, E.C., Candelli, M., Novi, M., Rigante, D., Cazzato, I.A., Ojetti, V., et al. (2006) Efficacy of Lactobacillus GG in Maintaining Remission of Ulcerative Colitis. Alimentary Pharmacology & Therapeutics, 23, 1567-1574. https://doi.org/10.1111/j.1365-2036.2006.02927.x
[15]
Shornikova, A.V., Casas, I.A., Isolauri, E., Mykkanen, H. and Vesikari, T. (1997) Lactobacillus Reuteri as a Therapeutic Agent in Acute Diarrhea in Young Children. Journal of Pediatric Gastroenterology and Nutrition, 24, 399-404. https://doi.org/10.1097/00005176-199704000-00008
[16]
Majamaa, H., Isolauri, E., Saxelin, M. and Vesikari, T. (1995) Lactic Acid Bacteria in the Treatment of Acute Rotavirus Gastroenteritis. Journal of Pediatric Gastroenterology and Nutrition, 20, 333-338. https://doi.org/10.1097/00005176-199504000-00012
[17]
Zhang, W, Azevedo, M.S.P., Gonzalez, A.M., Saif, L.J., Van Nguyen, T., Wen, K., Yousef, A.E. and Yuan, L. (2008) Influence of Probiotic Lactobacilli Colonization on Neonatal B cell Responses in a Gnotobiotic Pig Model of Human Rotavirus Infection and Disease. Veterinary Immunology and Immunopathology, 122, 175-181. https://doi.org/10.1016/j.vetimm.2007.10.003
[18]
Link-Amster, H., Rochat, F., Saudan, K.Y., Mignot, O. and Aeschlimann, J.M. (1994) Modulation of a Specific Humoral Immune Response and Changes in Intestinal Flora Mediated through Fermented Milk Intake. FEMS Immunology and Medical Microbiology, 10, 55-63. https://doi.org/10.1111/j.1574-695X.1994.tb00011.x
[19]
Olivares, M., Diaz-Ropero, M.P., Sierra, S., Lara-Villoslada, F., Fonolla, J., Navas, M., Rodriguez, J.M. and Xaus, J. (2007) Oral Intake of Lactobacillus fermentum CECT5716 Enhances the Effects of Influenza Vaccination. Nutrition, 23, 254-260. https://doi.org/10.1016/j.nut.2007.01.004
[20]
de Vrese, M., Rautenberg, P., Laue, C., Koopmans, M., Herremans, T. and Schrezenmeir, J. (2005) Probiotic Bacteria Stimulate Virus-Specific Neutralizing Antibodies Following a Booster Polio Vaccination. European Journal of Nutrition, 44, 406-413. https://doi.org/10.1007/s00394-004-0541-8
[21]
Kaila, M., Isolauri, E., Soppi, E., Virtanen, E., Laine, S. and Arvilommi, H. (1992) Enhancement of the Circulating Antibody Secreting Cell Response in Human Diarrhea by a Human Lactobacillus Strain. Pediatric Research, 32, 141-144. https://doi.org/10.1203/00006450-199208000-00002
[22]
Miura, K., Ishioka, M. and Iijima, K. (2017) The Roles of the Gut Microbiota and Toll-like Receptors in Obesity and Nonalcoholic Fatty Liver Disease. Journal of Obesity & Metabolic Syndrome, 26, 86-96. https://doi.org/10.7570/jomes.2017.26.2.86
[23]
Zhu, J., Yang, Q., Cao, L., Dou, X., Zhao, J., Zhu, W., Ding, F., Bu, R.-E., Suo, S., Ren, Y., et al. (2013) Development of Porcine Rotavirus vp6 Protein Based ELISA for Differentiation of This Virus and Other Viruses. Virology Journal, 10, 91. https://doi.org/10.1186/1743-422X-10-91
[24]
Azevedo, M.S.P., Yuan, L., Pouly, S., Gonzales, A.M., Jeong, K.I., Nguyen, T.V. and Saif, L.J. (2006) Cytokine Responses in Gnotobiotic Pigs after Infection with Virulent or Attenuated Human Rotavirus. Journal of Virology, 80, 372-382. https://doi.org/10.1128/JVI.80.1.372-382.2006
[25]
Wen, K., Azevedo, M.S.P., Gonzalez, A., Zhang, W., Saif, L.J., Li, G., Yousef, A. and Yuan, L. (2009) Toll-Like Receptor and Innate Cytokine Responses Induced by Lactobacilli Colonization and Human Rotavirus Infection in Gnotobiotic Pigs. Veterinary Immunology and Immunopathology, 127, 304-315. https://doi.org/10.1016/j.vetimm.2008.10.322
[26]
Isolauri, E., Joensuu, J., Suomalainen, H., Luomala, M. and Vesikari, T. (1995) Improved Immunogenicity of Oral D x RRV Reassortant Rotavirus Vaccine by Lactobacillus casei GG. Vaccine, 13, 310-312. https://doi.org/10.1016/0264-410X(95)93319-5
[27]
Zhang, W., Azevedo, M.S., Wen, K., Gonzalez, A., Saif, L.J., Li, G., Yousef, A.E. and Yuan, L. (2008) Probiotic Lactobacillus Acidophilus Enhances the Immunogenicity of an Oral Rotavirus Vaccine in Gnotobiotic Pigs. Vaccine, 26, 3655-3661. https://doi.org/10.1016/j.vaccine.2008.04.070
