bFGF对大鼠脑出血病灶周边脑组织细胞凋亡的影响及机制初探
The Influence and Regulatory Mechanism of the Basic Fibroblast Growth Factor (bFGF) on the Apoptosis of Nerve Cells Surrounding Intracerebral Hemorrhage (ICH) in Rat

DOI: 10.12677/JPS.2020.72002, PP. 7-12

Keywords: 碱性成纤维细胞生长因子，脑出血，p-AKT (Ser473)，细胞凋亡
Basic Fibreglass Growth Factor, Hemorrhage, p-AKT (Ser473), Apoptosis

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Abstract:

目的：观察碱性成纤维细胞生长因子(basic fibreglass growth factor, bFGF)对大鼠脑出血周边脑组织神经细胞凋亡的影响，并进行相关机制的初步探讨，为进一步深入了解bFGF对脑出血后神经细胞的保护机制的研究奠定基础。方法：通过脑内定位注射胶原酶，建立大鼠脑出血模型；使用假手术组和出血模型组作为对照，采用Zea Longa 5分制评分法对术后大鼠神经功能进行评价，出血模型建立成功的大鼠又分为对照组和bFGF治疗组，bFGF治疗组按8 ug/kg剂量肌内注射；各组分别于术给药后48小时取血肿旁大脑皮质，TUNEL法检测细胞凋亡数目，免疫组化检测p-AKT表达，并对结果进行统计分析。结果：大鼠脑出血模型构建成功；bFGF治疗组与对照组相比，Zea Longa评分显著降低，血肿旁大脑皮质凋亡细胞数量减少，p-AKT表达增高(P < 0.05)。结论：bFGF对大鼠脑出血后周边脑组织具有保护作用，其可能机制是通过提高大鼠脑出血周边脑组织p-AKT的表达来减少神经细胞凋亡数量，从而对脑出血后脑损伤具有保护作用实现。
Objective: To study the influence and regulatory mechanism of the basic fibroblast growth factor (bFGF) on the apoptosis of nerve cells surrounding intracerebral hemorrhage (ICH) in Rat. Methods: The rat model of experimental ICH was established by injection of collagenase VII into internal capsule of cerebrum. Then bFGF treatment group was injected with bFGF 8 vg/kg. After 48 h of treatment, we detected apoptosis of the cerebral cortex near the hematoma by TUNEL, and the phosphorylation level of AKT by immunohistochemistry. Results: Compared with control group, the apoptosis cells surrounding intracerebral hemorrhage of bFGF treatment group were decreased, and the phosphorylation level of AKT was enhanced (P < 0.05). According to Zea Longa method, the neurological score was decreased in the bFGF treatment group. Conclusion: bFGF could enhance the AKT phosphorylation level and reduce apoptosis of the cells surrounding intracerebral hemorrhage.

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