STUDY OF BIOLOGICAL EFFECTS WHEN EXPOSURE TO He-Ne LASER AND UVC RADIATION ON HEAD SPERM ABNORMALITIES IN MICE

In vitro, He-Ne laser show a modifying response of cells to ionizing radiations. So there is a need to show the effect of He-Ne laser (632.8 nm), Ultraviolet radiation UVC (250 nm) and He-Ne laser pre and post irradiation against the UVC radiation in vivo on sperm morphology of testis in Mice. In this study 100 albino male mice were divided into five groups, the first group is control which have (10) number of mice, the second group is Laser which have (27) number of mice were divided into three groups different time periods (5, 10, 15 min), the third group is Ultraviolet radiation (UVC) which have (9) number of mice and duration of exposure one hour, the fourth is group laser (5, 10 and 15 min) + UVC (1 hr.) which have (27) number of mice, with ？ hr. time interval between the two irradiations and the finally group is UVC (1 hr.) + laser (5, 10, 15 min) which have (27) number of mice, with ？ hr. time interval between the two irradiations was monitor the effect of radiation on mice according to the classification totals above after various time periods (7, 14, 21 days). Four sperm head abnormality features were found after each type of irradiation. But the He-Ne laser pre-irradiation against UVC irradiation lower the frequencies of sperm head abnormalities. Both UVC and He-Ne laser irradiations revealed a toxic effect on sperm head morphology, but the He-Ne laser was found less effective. The He-Ne laser per-irradiation show a protection properties, which appeared on both sperm head abnormalities and the protective properties were observed only when He-Ne laser irradiation was applied before the UVC irradiation