OPTIMIZATION OF GENOMIC DNA EXTRACTION ROUTINE FROM ROSEWOOD (DALBERGIA LATIFOLIA) AND BEN TEAK (LAGERSTROEMIA LANCEOLATA)

Dalbergia latifolia and Lagerstroemia lanceolata are two economically important and expensive timber species of tropical countries (India, Indonesia and Madagascar, Burma) are exploited. An efficient and on site detection and legal confirmation of these timbers is possible using molecular methods, but molecular tools have not been employed due to lack of knowledge of DNA extraction and species specific identification. Purified genomic DNA, required for many applications in molecular genetics is frequently more difficult to obtain from trees than most other crop or annual plants. Here we describe the essential steps of a rapid DNA isolation protocol that can be used for diverse timber species, which contains huge amount of secondary metabolites such as alkaloids, flavonoids, phenols, gummy polysaccharides, terpenes and quinones. This modified DNA extraction protocol is applicable for fresh and mature dried leaf samples. This customized procedure has improved the efficiency of genomic DNA by enhancing the yield as well as purity of DNA that would facilitate efficient characterization and validation of these timber species. The isolated DNA has been proved to be acquiescent to PCR amplification though ISSR and SSR primers