MULTIDRUG RESISTANT BACTERIA CREEPING INTO A NEWLY SET UP TEACHING HOSPITAL-TIME TO INTERVENE

Introduction: Antimicrobial Resistance (AMR) has been on continuous rise and drug resistant bacteria are the commonest etiology in hospitalized and community acquired infections. The objective of this study was to detect multidrug resistant organisms isolated from various clinical specimen, and their antibiotic profile. Materials and Methods: 750 Clinical samples were cultured; organisms were isolated and identified. Antibiotic susceptibility test was done based on Kirby Bauer disc diffusion method. ESBL production was tested by phenotypic double disc potentiation test. AmpC ？-lactamse production was tested by disc antagonism method. Detection of serine carbapenamases and MBLs was performed by Modified Carbapenem Inactivation method and EDTA Modified Carbapenem Inactivation method respectively as described by new CLSI guidelines. Among the Gram positive cocci, Methicillin and Inducible Clindamycin resistance was detected by cefoxitin disc diffusion and D-test respectively. Results: Among the resistant gram-negative bacteria, 33(10.9%) were ESBLs, 11(3.65%) Amp C, 3(1%) each were ESBL+Amp C, inducible AmpC and MBLs. Out of 150 resistant staphylococcal isolates, 103(68.6%) showed methicillin resistance and 26 (17.3%) showed inducible clindamycin resistance. Multidrug resistance (MDR) was observed in 4.8% of Gram negative and 2% of Gram positive bacteria. Extremely drug resistant bacteria (XDR) were found in 2% and 1% of Gram negative and Gram positive bacteria. Conclusion: Rising levels of AMR mandates routine detection of various types of resistance patterns. Routine detection of ESBLs, screening for Amp C beta lactamases, inducible Amp C, and confirmation of MBLs will help in providing authentic antibiotic susceptibility testing reports