Tumor cell-free DNA detection in CSF for primary CNS lymphoma diagnosis

MYD88 L265P quantification by ddPCR. Technical workflow for the CSF analysis and 2D ddPCR diagram of the fluorescence amplitude. Lower left quadrant contains the droplets with no MYD88 alleles; upper left contains droplets with MYD88L265P cfDNA; upper right contains droplets with both wild-type and mutant alleles; lower right contains droplets with MYD88 wild-type DNA. ddPCR results table for cell and cell-free CSF and brain biopsy, comparison with cytology and FCM. NA, not available; ND, not determined; NI, not interpretabl