Differential Expression of KCNJ12 Gene and Association Analysis of Its Missense Mutation with Growth Traits in Chinese Cattle

A central goal of livestock genomic study is to find causal genes underlying economic traits and identify effective variations which can be used as molecular markers for livestock breeding. The cattle KCNJ12 gene is an important candidate gene. To date, however, there have been no reports about the use of its missense mutation as a marker in cattle stature. In this study, missense mutation in KCNJ12 was firstly verified, which led to a change in its protein sequence. Further, a significant association was detected between the mutation of KCNJ12 and cattle stature, and we determined that the mutation in KCNJ12 could be used as a molecular marker in beef breeding programs. In addition, expression analysis of the KCNJ12 gene revealed high abundance in muscle and potential roles in bovine myocyte differentiation, which may be the subject of our future research