Grading Distress of Different Animal Models for Gastrointestinal Diseases Based on Plasma Corticosterone Kinetics

Animal welfare is an important aspect of biomedical research. Many regulations have been implemented to combine high quality of research with minimal harm to laboratory animals. These guidelines also demand a prospective severity assessment of each animal model. A comparison of distress between animal models could allow realistic harm and benefit analysis and an appropriate use of refinement methods. However, studies comparing distress between different animal models are still rare. One good parameter for analyzing distress is the concentration of the stress hormone corticosterone in the blood. Therefore, we compared the corticosterone kinetics of distinct gastrointestinal animal models. The aim of this study was to evaluate which parameter the highest corticosterone concentration or the duration of increased stress hormone level could be used to quantify distress. We observed a significant increase of corticosterone 30 min after stress induction in all animal models. However, the corticosterone kinetics differed between the distinct interventions. Both the absolute value and the duration of increased corticosterone level correlated directly with an assessed distress score. We conclude that both variables of corticosterone kinetics are valid parameters to compare distress between animal models