The ionophore antibiotic gramicidin A inhibits pancreatic cancer stem cells associated with CD47 down-regulation

Tumorsphere formation assay. a Representative images of tumor spheres in BxPC-3 and MIA PaCa-2 cells obtained before treatment (scale bar: 100 μm). b Representative images of BxPC-3 and MIA PaCa-2 tumorspheres obtained after the treatment with the indicated concentrations of GrA for 7 days (scale bar: 50 μm). c Statistical analysis of the number (% of control) of BxPC-3 and MIA PaCa-2 tumorspheres treated with GrA or Sal for 7 days, tumorsphere numbers were presented as mean？±？SD from 3 replicated wells. *P？<？0.05, **P？<？0.01, #P？<？0.05 vs contro