Mixed-lineage leukemia protein 2 suppresses ciliary assembly by the modulation of actin dynamics and vesicle transport

a Quantitative RT-PCR analysis of MLL2 mRNA expression in RPE-1 cells transfected with control or MLL2 siRNAs and serum-starved for 48？h. b Immunoblot analysis of H3K4me1 and β-actin levels in RPE-1 cells transfected with control (C) or MLL2 (M) siRNAs and serum-starved for 0, 12, 24, and 48？h. c–e Immunofluorescence images (c), percentage of ciliated cells (d, n？=？200), and ciliary length (e, n？=？80) for RPE-1 cells transfected with control or MLL2 siRNAs, serum-starved for 48？h, and stained with antibodies against γ-tubulin (red) and acetylated α-tubulin (green), and the DNA dye DAPI (blue). Scale bar, 10？μm. f–h Immunofluorescence images (f), percentage of ciliated cells (g, n？=？200), and ciliary length (h, n？=？50) for RPE-1 cells treated with the indicated concentrations of MTA, serum-starved for 48？h, and stained with antibodies against γ-tubulin (red) and acetylated α-tubulin (green), and DAPI (blue). Scale bar, 10？μm. i–k Immunofluorescence images (i), percentage of ciliated cells (j, n？=？100), and ciliary length (k, n？=？30) for RPE-1 cells transfected with the indicated siRNAs and plasmids, followed by serum starvation and staining with acetylated α-tubulin antibodies (red) and DAPI (blue). Scale bar, 10？μm. **P？<？0.01; ***P？<？0.001. Error bars indicate SE