Circadian control of stress granules by oscillating EIF2α

a The liver tissues from the wild-type (WT) mice harvested at ZT 5 and ZT 13 were labeled with anti-PABP1 (stress granule marker) antibody and revealed by immunofluorescence. Mice were intraperitoneally injected with saline or sodium arsenite (SA, 10？mg/kg) for 1？h before killing. Representative micrographs showing the presence of PABP1-positive stress granules (Scale bar？=？10？μm). b, c Quantification of all visible PABP1 puncta (b) or large size puncta (>0.5？μm, c) respectively (n？=？3 mice per group with 950–1000 cells examined per mouse. mean？±？S.E.M.; two-way analysis of variance (ANOVA) with Tukey’s multiple comparison *P？≤？0.05, ***P？≤？0.001