Characterization of CD44 intracellular domain interaction with RUNX2 in PC3 human prostate cancer cells

Characterizing the expression of CD44 in PCa cell lines. a. Real-time PCR analysis of CD44 expression in PC3 (lane 1), LNCaP (lane 2), and PCa2b (lane 3) cells. b. Immunoblotting (IB) analysis with an antibody to CD44 (top panel) and GAPDH (bottom panel). Equal amounts of protein lysates (40？μg) made from PC3 (lane 1), LNCaP (lane 2), PCa2b (lane 3), control HPR1 and RWPE1 (lane 4 & 5) cells were immunoblotted with CD44 antibody to detect total cellular levels. c. Equal amounts of PC3, LNCaP, PCa2b cells were immunoblotted with CD44 antibody. d. Equal amounts of PC3 (lane 1), PC3/AR+ (lane 2), and LNCaP (lane 3) were immunoblotted with CD44 antibody to detect total cellular levels. GAPDH was used as a loading control for real-time PCR and immunoblotting analysis (a-c). The results represent one of three separate experiments performed with the same result