Anti-apoptotic A1 is not essential for lymphoma development in Eμ-Myc mice but helps sustain transplanted Eμ-Myc tumour cells

Loss of A1 does not perturb lymphomagenesis in Eμ-Myc mice. a Kaplan–Meier survival curves for A1+/+ Eμ-Myc (n？=？27, median survival of 92 days), A1+/？ Eμ-Myc (n？=？64, median survival of 95 days) and A1？/？ Eμ-Myc (n？=？49, median survival of 94 days) mice. Data for A1-1 and A1-2 cohorts (Supplementary Figure S2a) have been pooled. The difference in survival between the genotypes was not significant (log？rank test). b Stacked bar graph showing the percentages of pro/pre-B (B220+sIg-), B (B220+sIg+) and mixed pre-B/B cell lymphomas observed for A1+/+ Eμ-Myc (n？=？21), A1+/？ Eμ-Myc (n？=？31) and A1-/- Eμ-Myc (n？=？36) mice. c Kaplan–Meier survival curves for pro/pre-B and B lymphoma development in A1+/+ Eμ-Myc (n？=？24), A1+/？ Eμ-Myc (n？=？34) and A1？/？ Eμ-Myc (n？=？40) mice. Only mice for which immunophenotyping was performed, or that survived until the endpoint, are included in these plots. No significant differences were apparent between genotypes in either the incidence (b) or kinetics (c) of pro/preB and B lymphomas. d Scatter plots of white blood cell counts (WBC) and weights of spleen, lymph nodes (inguinal, axillary and brachial lymph nodes) and thymus of autopsied sick A1+/+ Eμ-Myc, A1+/？ Eμ-Myc and A1？/？ Eμ-Myc mice that had reached ethical endpoint. Bars represent mean？±？SEM. No significant differences were found (Student’s T-test