A cytoskeleton structure revealed by super-resolution fluorescence imaging in inner ear hair cells

a Representative conventional epifluorescence vs. SIM fluorescence images of F-actin in the cuticular plates of OHCs from P12 mice (n？=？5 mice). Magnifications of the boxed region and intensity profiles from the corresponding lines are shown. b Representative SIM images of F-actin in the cuticular plates from P12 mice at the apex (n？=？3 mice), middle (n？=？3 mice) and base (n？=？3 mice) region of the cochlea. Intensity profiles along dashed lines are shown. x？ shows the average distance between two adjacent peaks. c Representative serial optical sections (interval？=？0.125？μm) of SIM images starting from the apical surface towards the deep region of the cuticular plate of OHC (P12, n？=？5 mice) along the Z-axis. Intensity profiles along the dotted lines are shown. Scale bars: 1？μm in upper panel a, 500？nm in lower panel a, 1？μm in b,