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- 2018
Phasing in on the cell cycleDOI: 10.1186/s13008-018-0034-4 Keywords: Protein phase separation, Oncogenic fusion, Protein aggregation, Cancer, Stress granules, Nucleolus, Centrosome Abstract: Identification of the phase separating proteome. Cleared cell lysate was incubated with poly-PR peptide to induce phase separation of cellular proteins. Phase separated proteins were precipitated by mild centrifugation and subjected to mass spectrometry. Identified proteins included stress granule factors and other membrane-less organelle components, but surprisingly as well proteins annotated as implicated in the regulation of the cell cycl
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