Distribution and abundance of Eimeria species in commercial turkey flocks across Canada

Diversity and regional abundance of Eimeria species infecting Canadian commercial turkey flocks are largely unknown. To address this paucity of data regarding coccidiosis and its distribution in Canada, fecal samples from turkey flocks (N = 39) representing 27 commercial farms [ON (n = 20), SK (n = 2), BC (n = 3), AB (n = 1), NS (n = 1)] were screened for coccidia. Identification of all Eimeria species present in each sample was accomplished using a nested-polymerase chain reaction (PCR) assay targeting the mitochondrial cytochrome C oxidase subunit I gene. Most samples (33/39) were Eimeria-positive with 6 Eimeria species identified by the nested-PCR assay (1 to 6 species/sample, average 3.2); 4 samples (4/39, > 10% of samples) contained all 6 species. Eimeria species were common and distributed widely in Canadian commercial turkey flocks. Turkeys reared using in-feed medication or live vaccination for coccidiosis control had similar Eimeria species diversity within individual flocks. These preliminary observations highlight that coccidiosis remains a concern for Canadian turkey producers