An Efficient and Reliable Assay for Investigating the Effects of Hypoxia/Anoxia on Drosophila

Design and set-up of the anoxia apparatus. A Schematic representation (upper) and image (lower) of the anoxia apparatus. Green arrows, N2 flow direction; red arrows and dots, extra gas discharge from the sealed anoxic chamber. B (i) Flies in 1.5 mL EP tubes with the lid removed and replaced with gauze; (ii) EP tubes upside down in holes of the 2-layer platform