Manipulation of dorsal raphe serotonergic neurons modulates active coping to inescapable stress and anxiety-related behaviors in mice and rats

srTPH2 and smTPH2 LVVs efficiently transduced serotonergic neurons in the DRN. a, b Rats were stereotaxically injected with srTPH2:Venus, srTPH2:ChETA or srTPH2:eArchT in the DRN. a One week after injection, coronal sections containing the DRN were prepared and stained by anti-GFP and anti-TPH antibodies. Stained sections were imaged using confocal microscopy. Some sections were left unstained in order to assess the expression levels of the transgenes (Venus or eYFP). Representative images from at least three rats are shown. Scale bars？=？20？μm (GFP, TPH, Merge) or 200？μm (Fluorescence). b One week after injection of srTPH2:ChETA or srTPH2:eArchT, 200-μm-thick coronal slices were prepared. The cells with eYFP fluorescence were analyzed by whole cell patch clamp. Blue light (20 pulses, 20？Hz, 5？ms duration) or green light (1 pulse, 0.5？s duration) was applied through fiber optics to illuminate patched cells. Representative traces of current-clamp recording (upper panel) and voltage-clamp recording (lower panel) are shown. Similar results were obtained in two (ChETA) and three (eArchT) rats. c, d Mice were injected with smTPH2:Venus, smTPH2:ChETA, or smTPH2:eArchT in the DRN. c One week after injection, coronal sections were prepared and processed as described in (a). Representative images from at least three mice are shown. Scale bars？=？20？μm (GFP, TPH, Merge) or 200？μm (Fluorescence). d One week after injection of smTPH2:ChETA or smTPH2:eArchT, 200-μm-thick coronal slices were prepared. The cells with eYFP fluorescence were analyzed by whole cell patch clamp. Blue light (20 pulses, 20？Hz, 5？ms duration) or green light (1 pulse, 0.5？s or 1？s duration) was applied through fiber optics to illuminate patched cells. Representative traces of current-clamp recording (upper panel) and voltage-clamp recording (lower panel) were shown. Similar results were obtained in eight (ChETA) and three (eArchT) mic