Up-Regulation of Akt and Nav1.8 in BmK I-Induced Pain

Expression patterns of Akt,p-Akt, and Nav1.8 in DRGs after BmK I injection. A Representative Western blots of Akt, p-Akt, and Nav1.8 immunoreactivity at different time points following intraplantar injection of BmK I. B–G Quantification of band intensity for the ipsilateral (B, D, F) and contralateral (C, E, G) sides. The fold change for the density of the Akt, p-Akt, and Nav1.8 bands was calculated after normalization to control. H, I. Changes in the mRNA expression levels of Nav1.8. J Co-localization of Nav1.8 and Akt with CGRP neurons. K, L Ipsilateral (K) and contralateral (L) quantification of the co-localization in Nav1.8-positive cells. M, N Ipsilateral (M) and contralateral (N) quantification of the co-localization in pAkt-positive cells. *P？<？0.05, **P？<？0.01, and ***P？<？0.001 (n？=？3) compared with control groups. All data are presented as mean？±？SEM