Genomic and expression profiling reveal molecular heterogeneity of disseminated tumor cells in bone marrow of early breast cancer

DTCs from bone marrow of early breast cancer patients were enumerated and isolated for downstream molecular profiling. a Enumeration and isolation of DTCs using a two-step process involving immunomagnetic enrichment and flow cytometry or fluorescence-activated cell sorting (IE/FACS). b Clinical characteristics of 71 patients from whom DTCs were enumerated. Each column represents a patient. c–d Comparison of DTC/mL between groups based on patient treatment and nodal status (also see Supplementary Fig. 1 for extended analysis