A platform for phenotypic discovery of therapeutic antibodies and targets applied on Chronic Lymphocytic Leukemia

Schematic outline of the methods included and the number of antibodies analyzed in the various steps of the CLL study. A CLL-specific antibody pool was generated by differential cell panning and individual soluble antibodies in scFv format were screened for cell binding in flow cytometry, FC, and fluorometric microvolume assay technology, FMAT. Clones binding specifically to CLL cells were DNA sequenced and unique clones were clustered based on cell binding pattern analysis. Several clones from each cluster were functionally tested in hIgG1 format in PCD and ADCC assays. Targets were identified for a subset of clones followed by in vivo testing in a PDX mode