Expression of lncRNA MIR222HG co-transcribed from the miR-221/222 gene promoter facilitates the development of castration-resistant prostate cancer

a H3K4Me3, H3K4Me2, and H3K27Ac marks in LNCaP and LNCaP-Abl. Top panel, the diagram of miR-221/222 gene and MIR222HG loci in Chr. Xp11. 3. All CHIP-seq data were documented in www.cistrome.org. b Upper panel, H3K4Me3 marks on seven cell lines from ENCODE hg19; lower panel, H3K27Ac marks on seven non-PCa cell lines (GM12878, Hi-hESC, HSMM, HUVEC, K562, NHEK, and NHLF) from ENCODE hg19 (www.genome.ucsc.edu). c Characterization of a promoter region at the 5′ region of the MIR222HG gene. Left panel, fragments of the promoter region were cloned into the upstream of a luciferase reporter gene. Right panel, promoter luciferase reporter assays in LNCaP-Abl cells. Relative luciferase activities were calculated in relative to the vector control (Renilla luciferase vector without a promoter) whose activity was arbitrarily set as