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OALib Journal期刊
ISSN: 2333-9721
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-  2017 

Standardization of DNA Residual Quantification Method of Vero Cell Rabies Vaccine for Human Use

DOI: 10.2174/1874104501711010066

Keywords: Real time PCR, Residual DNA, Vero cells, Antirrabic vaccine, Betapropiolactone

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Abstract:

Normalize the quantification of residual DNA from Vero cells in the rabies vaccine for use in human VAHV I, by quantitative PCR in real time and the design of primers that amplified, highly repetitive sequences of Cercopithecus aethiops and a constitutive gene according to sequences reported in the GenBank and quantifying the residual DNA in the vaccine VAHV I in three consecutive batches according to the standard set by the World Health Organization

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