|
- 2017
Suppl-1, M2: Deep Sequencing Details the Cross-over Map of Chimeric Genes in Two Porcine Reproductive and Respiratory Syndrome Virus Infectious ClonesDOI: 10.2174/1874357901711010049 Keywords: Cross-over map, Deep sequencing, Green fluorescent protein, Infectious clone, Porcine reproductive and respiratory syndrome virus (PRRSV), Viable recombinant Abstract: Recombination is an important contributor to the genetic diversity of most viruses. A reverse genetics system using green fluorescence protein (GFP)- and enhanced GFP (EGFP)-expressing infectious clones was developed to study the requirements for recombination. However, it is still unclear what types of cross-over events occurred to produce the viable offspring
|