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- 2017
Effects of light-emitting diode (led 640nm) on human gingival fibroblasts: a comparative in vitro studyDOI: 10.11138/orl/2017.10.2.151 Keywords: light-emitting diode, human gingival fibroblasts, primary fibroblasts cell culture, Real Time Polymerase Chain Reaction Abstract: The light-emitting diodes (LEDs) have been applied in oral surgery for tissue stimulation and wound healing. Several Authors have highlighted that fibroblasts subjected to phototherapy have an increased viability, proliferation, biomodulation of inflammatory cytokines and genes expression. It remains to be determined which are the best irradiation parameters (energy, wavelength, power) for each type of cell in order to obtain the best bio-stimulation. The aim of this study was to investigate the effects of LED irradiation on primary human gingival fibroblast cells (HGF) on DSP, ELN, HAS1, ELANE, HYAL1, RPL13 genes activation using Real Time PCR. These genes activation is directly connected with elastin protein production and HGF functionality
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