System immunology-based identification of blood transcriptional modules correlating to antibody responses in sheep

Neutralizing antibodies, haptoglobin and differentially expressed genes in PBMC induced by vaccination. a Study design. b Serum neutralizing antibody titers following vaccination of sheep with antigen in phosphate buffered saline (PBS), antigen formulated in liposomes (L) and antigen formulated in liposomes with TLRL (L(TLRL)). c Vaccine-induced haptoglobin responses. d Vulcano plots showing differentially expressed genes which were up- or downregulation on day 3 (D3) and day 7 (D7) compared with before immunization, respectively. Green dots represent significant genes (p adjusted？<？0.05). Digits in the plots indicate the number of significantly down or upregulated genes. In b and c, statistical significant differences between two groups were calculated using unpaired two-way ANOVA with repeated measures followed by Tukey’s multiple comparisons test (***p？<？0.001; **p？<？0.002; *p？<？0.033