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- 2019
Single dose of a rVSV-based vaccine elicits complete protection against severe fever with thrombocytopenia syndrome virusDOI: 10.1038/s41541-018-0096-y Abstract: Characterization of the rVSV viruses. a Schematic diagrams show the organization of reporter and viral proteins in rVSV and rVSV-eGFP vectors. b Examining the incorporation of SFTSV Gn/Gc into the rVSV vectors. Rescued rVSV-SFTSV/AH12-GP, rVSV-eGFP-SFTSV/AH12-GP, and rVSV-eGFP-SFTSV/YG1-GP in the supernatant of 293?T cells were pelleted by ultracentrifugation and blotted with polyclonal antibodies against SFTSV Gn and Gc. Control (Con.) is the supernatant of non-transfected 293?T cells. Full scans of the blots are in Supplementary Fig 3. All blots derived from the same experiment and were processed in parallel. c Examining the expression of SFTSV Gn/Gc in rVSV-infected cells. Vero cells infected with rVSV-SFTSV/AH12-GP, rVSV-eGFP-SFTSV/AH12-GP, or rVSV-eGFP-SFTSV/YG1-GP were lysed and blotted as above. Control (Con.) is the noninfected cells. Full scans of the blots are in Supplementary Fig 3. All blots derived from the same experiment and were processed in parallel. d Neutralizing response of the rVSV-eGFPs to the serum from a convalescent SFTS patient as measured on Vero cells via an FRNT assay. e Plaque formation of rVSVs in Vero cells. Vero cells were infected with indicated viruses and stained by crystal violet 48?h (rVSV-G) or 96?h (all the other viruses) post infection. Scale bars indicate 500?μm. Data are representatives of three independent experiments. f Growth curves of rVSV viruses. Vero cells were infected by the rVSV viruses at an M.O.I. of 0.01 and virus titer in the supernatant was measured at the indicated time points post infection via a plaque assay on Vero cells. Each bar shows 95% cl. g Cytopathic effects of rVSV-SFTSV/AH12-GP. Vero cells were infected by the indicated viruses at an M.O.I. of 0.1 and bright-field images were taken 24?h post infection. Control (Con.) is the noninfected cells. Scale bars indicate 125?μm. Above data are representatives of three independent experiment
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