Single-base resolution methylome analysis shows epigenetic changes in Arabidopsis seedlings exposed to microgravity spaceflight conditions on board the SJ-10 recoverable satellite

Hardware and experimental procedures used in the experiment on board the SJ-10 recoverable satellite. a Appearance of the hardware used in the spaceflight experiment. The equipment functioned as a housing for the culture chambers. b Distribution of internal components of the hardware. The hardware includes culture chambers, a pump support system, a canal system and a fixative unit connected to the culture chambers. c Arabidopsis seedlings were grown in culture chambers during the space experiment. d Schematic view of the Arabidopsis seedlings on board the SJ-10 satellite during spaceflight and on the ground. Arabidopsis seedlings in culture chambers were transferred from Petri dishes 3 days before launching. Seedlings in culture chambers installed in cultivation unit 1 were grown for 60？h under microgravity conditions and fixed in space using RNAlater？. The seedlings in the culture chamber installed in cultivation unit two grew for 11 days under microgravity; after this time, they were still alive and returned with the satellite. The g-profile during SJ-10 satellite in orbit is described in the references.15,16 The g-profile during launch was as follows: the first-level maximum static overload was 4.8？g in flight for 150？s; the second-level maximum static overload was 6.0？g in flight for 180？