Recovery of stem cell proliferation by low intensity vibration under simulated microgravity requires LINC complex

Simulated microgravity (sMG) decreases cell proliferation without affecting cell viability. a MSCs were subjected to simulated microgravity (sMG) via rotation at a constant 15 RPM for 3 days inside 12.5？cm2 sealed flasks. LIV was applied twice daily at 2？h intervals for 3 days outside the incubator at 0.7？g’s and 90？Hz for 20？min. During LIV, all samples were taken out but only sMG？+？LIV group was vibrated. Samples were collected for analysis 24？h after last LIV treatment. b Comparing the cell proliferation and dead cell to live cell ratio using microscopy indicated less cells in field of view (10？×), flow cytometry showed that sMG decreases cell proliferation by 2.63-fold (p？<？0.01, n？=？6/group). Cell death was not different between difference Ctrl and sMG samples (n？=？6/group). Group comparisons were made using unpaired T-test (b). *p？<？0.05, **p？<？0.01, ***p？<？0.001, against control and each othe