Spaceflight-induced alternative splicing during seedling development in Arabidopsis thaliana

Detection of spaceflight-induced alternatively spliced transcript isoforms. a Bioinformatics pipeline utilized to identify all transcript isoforms and significantly differentially spliced genes present in the original RNA sequencing dataset. Steps inside the dashed outline were performed on each individual replicate. See Methods for more detail on gtf merging. b Comparison of alternatively spliced isoforms present or absent in spaceflight in both ecotypes and developmental ages tested. c Count of differentially alternatively spliced genes and the associated count of transcript isoforms observed in each condition. d Comparison of the differentially alternatively spliced genes observed in the Columbia-0 (Col-0) or the Wassilewskija (WS) ecotype background. Differentially alternatively spliced genes observed at both 4- and 8-day time points in either ecotype background were pooled for this analysis. (C Columbia-0, W Wassilewskija, 4 4 days of age, 8 8 days of age. C4 Columbia-0 4？days of age, W8 Wassilewskija 8？days of age