Adaptive metabolic pattern biomarker for disease monitoring and staging of lung cancer with liquid biopsy

Effects of oxygen on human PBMCs spiked with a tumor cell line. Flow cytometry of PBMCs obtained from human blood from a healthy donor sample spiked with A549 cells (1:100 ratio, cancer:healthy) as a function of the oxygen conditions. a normoxia; b hypoxia; and c hyperoxia. d Average 2NBDG fluorescence emission per cell for the different oxygen conditions. e Ratiometric differences in 2NBDG fluorescence emission for cancer cells (A549) and PBMCs (lymphocytes and monocytes) under the three oxygen conditions. FSC: forward-scattered ligh