CRISPR/Cas9 – An evolving biological tool kit for cancer biology and oncology

CRISPR/Cas9-based gene modification. Common methods of delivering the CRISPR system include a plasmid-based method and Cas9 protein complex with sgRNA or RNP. After the sgRNA binds to the target site of genomic DNA, the Cas9 protein creates a DSB around the PAM site. Random indels or precise modifications introduced into the genomic DNA by the NHEJ or HDR pathwa